中文摘要：

为研究家蚕富集1-脱氧野尻霉素（1-deoxynojirimycin,DNJ）的机制,本实验分析了不同品种家蚕体内DNJ含量并研究其与蚕体内糖苷酶的关系。选择了25个不同的家蚕品种,用相同条件的桑叶饲喂不同品种的家蚕,采用柱前衍生高效液相色谱法分析不同品种蚕体DNJ含量并研究其与体内蔗糖酶、乳糖酶及淀粉酶3种糖苷酶活性之间的相关性。结果表明：不同品种家蚕体内DNJ含量差异显著,DNJ含量最高种是最低种的7倍,蚕体DNJ含量与体内的蔗糖酶、乳糖酶等二糖酶活力成显著负相关,与淀粉酶的活力相关性相对较弱。

英文摘要：

In order to reveal the mechanism of 1-deoxynojirimycin （DNJ） enrichment in silkworm （Bombyx mori L.）, westudied and analyzed the correlation between DNJ content and glucosidase activities in silkworm. Towards this goal, 25strains of silkworms were selected and raised with the same mulberry leaves. Pre-column derivatization high performanceliquid chromatography （HPLC） was applied to determine the DNJ content of different strains of silkworms, and the activitiesof 3 major glucosidases （sucrase, lactase and amylase） were measured, respectively, for the analysis of correlation betweenDNJ content and glucosidase activities in silkworms. Our results indicated that different strains of silkworms had significantdifferences in DNJ content, and the highest DNJ content （which was found in the strain 2042, 0.56%） was 7 times higherthan the lowest one （in ‘DongTing × BiBo’, 0.08%）. DNJ content had significantly negative correlations with the activity ofeither of two disaccharidases, sucrase and lactase, and had a relatively weaker correlation with amylase activity.