中文摘要：

目的：建立千里光药材中金丝桃苷的RP-HPLC含量测定方法。方法：采用Shiseido Capcell Pak MGⅡC18色谱柱（4.6mm×250mm,5μm）,流动相为乙腈-0.2%醋酸（18：82）,流速1.0mL.min-1,检测波长360nm,柱温35℃。结果：金丝桃苷进样浓度在0.9980～199.6μg.mL-1范围内呈现良好线性关系（r=0.9994,n=6）;25%,50%,75%,100%,150%浓度水平下加样回收率在99.7%～103.5%之间,RSD〈2.0%;重复性及精密度良好,且具有较好的色谱条件耐用性。结论：所建立HPLC方法灵敏、准确,重现性好,适用于千里光药材中金丝桃苷的含量测定,可用于千里光药材的质量控制,为千里光药材2010年版中国药典的质量标准研究奠定基础。

英文摘要：

Objective：To develop an RP-HPLC method for quantitation of hyperoside in Herba Senecionis Scandentis.Methods：Chromatography was performed on a Shiseido Capcell Pak MG Ⅱ C18 （4.6 mm×250 mm,5 μm） column at 35 ℃ using an isocratic elution of acetonitrile-0.2% acetic acid in water（18：82） at the flow rate of 1.0 mL·min-1.And detection wavelength was 360 nm.Results：Good linearity was obtained over the range of 0.9980-199.6 μg·mL-1（r=0.9994,n=6）.And recoveries at different concentration levels were between 99.7% and 103.5% with RSDs less than 2.0%.Good reproducibility and precision as well as ruggedness were also achieved.Conclusion：The new developed RP-HPLC method is sensitive,accurate and reliable enough for the quantitation of hyperoside in Herba Senecionis Scandentis thus can be used for the quality evaluation on Herba Senecionis Scandentis.