中文摘要：

为评估纳米羟基磷灰石颗粒负载p53基因对肝癌干细胞的抑制效果,采用化学沉淀法制备羟基磷灰石,对其进行表征,并检测其生物相容性。通过成球培养法获得肝癌干细胞,用经聚乙烯亚胺（PEI）修饰的纳米羟基磷灰石颗粒（HP）负载p53基因（HP-p53）处理肝癌干细胞,用MTT法、hoechst 33258染色综合评估杀伤效果。结果表明：所制备的纳米颗粒主要成分和晶型为羟基磷灰石,呈短棒状,长径为20-50nm,短径约为20nm,并具有良好的生物相容性和降解性;HP-p53处理后的肝癌干细胞存活率显著下降;用Hoechst 33258染色观察到HP-p53处理后的肝癌干细胞细胞核出现高亮光斑,细胞密度降低,表明所制备的纳米羟基磷灰石负载p53基因对肝癌干细胞具有显著的抑制效果。

英文摘要：

To evaluate the inhibition effect of p53 gene loaded by hydroxyapatite nanoparticles on hepatic cancer stem cells （CSCs） , the hydroxyapatite nanoparticles were prepared by chemical precipitation method and then characterized to test biocompatibility. Hepatic cancer stem cells were obtained in spheric culture condition. p53 gene loaded by hydroxyapatite nanoparticles modified by polyethylenimine （PEI） （HP-p53） was used to treat hepatic cancer stem cells. The inhibition effect was tested by MTT assay and Hoechst 33258 staining. The experiments suggest that HP is short rod-like nanoparticle with length ranged from 20 to 50 nm and width of approximately 20 nm and its main component is hydroxyapatite. HP has excellent biocompatibility and degradability. The survival rate of hepatic cancer cells treated by HP- p 53 decreased significantly. Hoechst 33258 staining results shows that bright spots appeared in the hepatic cancer stem cell nucleus, and cell density decreased. This indicates p5 3 gene loaded by hydroxyapatite nanoparticles has significant inhibition effect on hepatic cancer stem cells.