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中文摘要：
Toll样受体（TLRs）及其信号传导是极其重要的免疫信号传导通路，在先天性免疫中发挥重要作用。本课题采用EST、同源克隆、Genome Walking和RACE等技术从栉孔扇贝中克隆TLRs及其信号传导通路中关键因子（如Myd88、TRAF6和Cactus等）的基因，确定扇贝中存在Myd88依赖信号传递途径。使用Northern Blot、Weatern Blot、Real time RT-PCR以及基因芯片等技术检测这些基因及一些效应因子（如抗菌肽、溶菌酶和酪氨酸酶等）基因在不同类型微生物或相应病原相关分子模式（PAMPs）刺激下的表达特征，同时检测扇贝个体或原代培养的血淋巴细胞中部分免疫学和免疫化学指标的变化，明确TLR信号通路对后续免疫应答反应的诱导和激活作用，进而深入了解扇贝免疫防御的机制，丰富和发展海洋无脊椎动物免疫学的内容，亦为贝类病害防治提供新的思路
结论摘要：
英文主题词Chlamys farreri; Toll; signal pathway; gene cloning and expression
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期刊论文
Molecular cloning and immune responsive expression of a novel C-type lectin gene from bay scallop Ar
Molecular cloning and responsive expression to injury stimulus of a defender against cell death 1 (D
The expression of peptidoglycan recognition protein-S1 gene in the scallop Chlamys farreri was enhan
The polymorphism of lysozyme gene in Zhikong scallop (Chlamys farreri) and its association with susc
Identification and expression of TRAF6 (TNF receptor-associated factor 6) gene in Zhikong Scallop Ch
Alteration of metallothionein mRNA in bay scallop Argopecten irradians under cadmium exposure and ba
Expressed sequence tags from the zhikong scallop (Chlamys farreri): Discovery and annotation of host
The cDNA cloning and mRNA expression of cytoplasmic Cu, Zn superoxide dismutase (SOD) gene in scallo
Molecular cloning and characterization of a short type peptidoglycan recognition protein (CfPGRP-S1)
Loss of allele diversity in introduced populations of the hermaphroditic bay scallop Argopecten irra
Identification and characterization of a myeloid differentiation factor 88 (MyD88) cDNA from Zhikong
Molecular cloning and expression of a Toll receptor gene homologue from Zhikong Scallop, Chlamys far
Molecular cloning and mRNA expression of peptidoglycan recognition protein (PGRP) gene in bay scallo
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