中文摘要：

目的探讨焦炉工人HSP70基因多态性与外周血淋巴细胞凋亡率的关系。方法于2008年7—8月，在某焦化厂选取341名工人作为研究对象，按照工人班后尿1-羟基芘（1-OHP）的浓度，分为低暴露组（〈1．52μmol／molor）、中暴露组（1．52～3．88Ixmol／molor）、高暴露组（〉3．88μmol／molor）。采用流式细胞仪测定淋巴细胞凋亡率，以限制性片段长度多态性（PCR—RFLP）方法分析焦炉作业工人外周血淋巴细胞HSP70-J基因G190C、HSP70—2基因A1267G和HSP70-horn基因T2437C基因多态性。结果携带HSP70-hom基因T／C＋C／C基因型中、高暴露组工人淋巴细胞早期凋亡率均高于低暴露组（P〈O．05）。携带HSP70-JG／C＋C／C、HSP70-2A／G＋G／G和HSP70-homT／C＋C／C基因型高暴露组工人淋巴细胞早期凋亡阳性率均高于低暴露组fP〈0．05）。HSP70—2基因A／G＋G／G基因型与高暴露环境之间存在次相乘模型的正向交互作用（7〉1，OR．t=11．273，95％C，=2．244—56．641）；HSP70-hom基因T／C＋C／C基因型与高暴露环境之间存在超相乘模型的正向交互作用（7〉1，ORe=3．718，95％CI=1．293～10．693）。低、中、高暴露组GAC、CGT单倍型者淋巴细胞早期凋亡阳性率逐渐增加（P趋势〈0．05）。结论HSP70—2基因A／G＋G／G基因型、HSP70-horn基因T／C＋C／C基因型与高暴露环境的交互作用可增加淋巴细胞凋亡的危险度。GAC和CGT单倍型是淋巴细胞早期凋亡阳性率增加的危险因素。

英文摘要：

Objective To study the feasibility of HSP70 genes polymorphisms as biological marker of lymphocytes in peripheral blood of the coke-oven workers injury susceptibility by investigating the relationship between HSPTO genes polymorphisms and lymphocyte apoptosis. Methods A total of 341 coke-oven workers were investigated in a eoking plant by the questionnaires from July to August,2008. 1-hydroxypyrene （1-OHP）concentration in worker＇s urine after work was determined by high performance liquid chromatography. Subjects were divided into low （〈1.52 μmol]mol cr）,medium （1.52- 3.88μmol/mol cr） and high exposure group（〉3.88 μmol/mol er） according to the 1-OHP concentration. Lymphocyte apoptosis in peripheral blood was determined using the flow cytometry. HSP70-1 G19OC,HSP70-2 A1267G,HSP70-hom T2437C genotypes were analyzed by the polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） technique. Results The lymphoeytes apoptosis rates in moderate and high exposure groups were significantly different compared with low exposure group in HSP70-hom T/C＋C/C genotypes （P〈0.05）. Gene potymorphisms analysis showed that there were significantly difference of lymphoeytes apoptosis positive rates between high exposure group and low exposure group among HSP70-1 G/C＋ C/C, HSP70-2 A/G＋G/G and HSPTO-hom T/C＋C/C genotypes （P〈0.05）. Interactions between genes and environment factors showed different effects on lymphocytes apoptosis rates increased. S!gnificant positive-interaction was observed between HSP70-2 A/G＋G/G genotype and high exposure environmental （＂/〉1,0R,s=l 1.273,95%CI=2.244-56.641）, which appeared to act in a sub-multiplicative model. There was also observed significant positive-interaction between HSP70-horn T/C ＋C/C genotype and high exposure environmental （γ〉1,OR,,=3.718,95%CI=1.293-10.693）, which appeared to act in a super- multiplieative model. The positive rate of lymphocyte apoptosis in the low,moderate and high exposure group wor