中文摘要：

目的 探讨热休克蛋白70（HSP70）基因多态性与焦炉工人肺通气功能损伤的关系.方法 选取某焦化厂183名焦炉作业工人（暴露组）及143名无焦化作业史的辅助工种工人（对照组）为研究对象.使用统一的调查表收集个人基本信息.便携式肺功能仪测定肺活量（VC％）、用力肺活量（FVC％）、最大通气量（MVV％）、第1秒用力呼气容积（FEV10％）、第1秒用力呼气容积与用力肺活量比值（FEV1.0/FVC％）等肺通气功能指标.采用限制性片段长度多态性（PCR-RFLP）方法分析焦炉作业工人HSP70-1 G190C、HSP70-2 A 1267G和HSP70-hom T2437C基因多态性.运用PHASE 2.0软件计算单倍型.结果 焦炉工人肺通气功能指标VC％、FVC％、MVV％、FEV10％均显著低于对照组,差异有统计学意义（P＜0.05）;暴露组HSP70-1、HSP70-2、HSP70-hom各基因型肺通气功能指标VC％、FVC％、MVV％、FEV10％均低于对照组;暴露组中HSP70-hom T/C基因型FVC％低于T/T基因型,MVV％低于T/T、C/C基因型,差异有统计学意义（P＜0.05）,HSP70-1、HSP70-2各基因型携带者的肺功能指标的差异均无统计学意义（P＞0.05）;暴露组HSP70-1、HSP70-hom各基因型携带者的肺通气功能障碍率的差异有统计学意义（P＜0.05）;与野生型比较,暴露组携带HSP70-1 G/C基因型与HSP70-hom T/C基因型者肺通气功能障碍率的调整OR（95％CI）分别为2.516（1.012-6.252）和2.284（1.033-5.053）.携带CGT单倍型者人群通气功能障碍率显著高于对照组,差异有统计学意义（P＜0.05）.结论 焦炉作业可致焦炉工人肺通气功能损伤,具有HSP70-1 G/C基因型、HSP70-hom T/C基因型和CGT单倍型的焦炉工人易感肺通气功能损伤.焦炉工人HSP70-1和HSP70-hom基因多态性与肺通气功能损伤相关联.

英文摘要：

Objective In this study,we investigate the relationship between HSP70 and lung function injury.To study on the feasibility of HSP70 genes polymorphisms as biological marker of the damage of pulmonary dysfunction susceptibility.Methods 183 cock-oven workers were selected as exposure groups and 143 workers unexposed workers were selected as control groups.We investigated their general information with uniform questionnaire.Pulmonary dysfunction indicators were determined using portable spirometer.HSP70-1 G190C,HSP70-2 A1267G,HSP70-hom T2437C genotypes were analyzed by using the polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） technique.The haplotypes were calculated using PHASE 2.0 software.Results VC%,FVC%,MVV%,FEV1.0% in exposed group were lower than in non-exposure group,the difference were significantly （P〈0.05）.VC%,FVC%,MVV%,FEV1.0% in exposed group with HSP70-1,HSP70-2,HSP70-hom genotypes were lower than in non-exposure group （P〈0.05）; FVC% in exposed group with HSP70-hom T/C genotypes were lower than that with HSP70-hom T/T genotypes,MVV% were lower than that with HSP70-hom T/T,C/C genotypes.There＇s no difference in pulmonary dysfunction index of HSP70-1,HSP70-2 genotypes （P〉0.05）,but significant difference between the exposed group with HSP70-1,HSP70-hom genotypes; The adjust OR（95%CI） of exposed group with HSP70-1 G/C genotypes and HSP70-hom T/C genotypes were 2.516 （1.012 -6.252） and 2.284（1.033-5.053）.Exposed group with CGT haplotype pulmonary dysfunction were significantly higher than in non-exposure group （P〈0.05）.Conclusion Coke oven exposure may increase pulmonary dysfunction injury,Coke oven workers who have the HSP70-1 G/C genotypes,HSP70-hom T/C genotypes and CGT haplotypes may increase the susceptibility of pulmonary dysfunction.There must be some relationship between HSP70-1,HSP70-hom gene polymorphisms and lung function injury of Cock-oven Workers.