采用表面光接枝改性,以二苯甲酮为光引发剂,在聚丙烯膜表面分别接枝一层聚丙烯酸(PAA)、聚丙烯酰胺(PAM)和马来酸酐(MAH),然后分别在上述接枝层表面对牛血清白蛋白(BSA)进行固定。采用称重法、付立叶红外光谱仪、水接触角测试仪、紫外-可见光谱等测试手段对表面改性效果和固定的蛋白量进行表征。结果表明,在经MAH、AA和AM接枝改性过的聚丙烯膜表面可以固定BSA。而且固定效果率比较为:MAH〉AA〉AM。
Layers of poly (acrylic acid) (PAA), polyacrylamide (PAM) and maleic anhydride(MAid) have been synthesized on polypropylene (PP) films by a surface photografting modification method using benzophenone (BP) as photoinitiator. Bovine serum albumin (BSA) was subsequently grafted onto these films. The effect of the surface modification and protein adsorption was determined by weight changes, FTIR-ATR, water contact angle measurements and UV-visible spectroscopy. The extent of grafting of BSA on the modified PP films decreased in the order PP-MAH 2〉 PP-AA 2〉 PP-AM.