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光引发淀粉-AM／AA反相乳液接枝聚合——光引发剂的影响

ISSN号：1000-7555
期刊名称：《高分子材料科学与工程》
时间：0
分类：Q966[生物学—昆虫学]
作者机构：[1]北京化工大学生命科学与技术学院,北京100029
相关基金：国家自然科学基金项目（50433040）

关键词：金特异结合肽, BAC-TO-BAC, 融合表达, 表面展示, 重组杆状病毒, 纳米复合体, Au-binding peptide, Bac-to-Bac, fusion expression, surface display, recombinant baculovirus, nanocomposite

中文摘要：

纳米金在生物探针方面的应用技术是重要的研究方向,这一技术的关键在于纳米金与生物分子有效的结合,本文通过基因重组策略,研究杆状病毒表面展示技术与金特异结合肽介导的固定化方法联用以构建生物-无机复合材料的可行性。利用Bac-to-Bac杆状病毒表达系统将合成的金特异结合肽基因融合到杆状病毒BmNPV囊膜糖蛋白gp64的N端信号肽与成熟蛋白之间构成转移质粒载体,经过转座得到重组穿梭质粒pFB-gp64-Au,转染Sf9昆虫细胞后收获多角体启动子控制下表达融合蛋白的重组AcMNPV。同时采用硼氢化钠还原法制备得到胶体金,进行组建病毒-胶体金复合结构的初步研究。结果表明,成功构建的重组穿梭质粒转染细胞后得到囊膜表面经金无机结合肽修饰的AcMNPV重组病毒,并通过透射电子显微镜（transmission electron microscopy,TEM）观察到由金特异结合肽所介导的病毒-胶体金的复合结构。这项研究有助于进行生物-无机复合体系构建及纳米材料在生物学领域应用的研究。

英文摘要：

The application of nanogold in bioprobe technology is a major research field,which mainly depends on the effective binding of biomolecules with gold nanoparticles.Using the strategy of gene recombination,we studied the feasibility of combining baculoviral surface display with peptide-mediated immobilization to make bio-inorganic composites.A gene encoding an Au-specific binding peptide （Au-bp） was synthesized and fused to the N-terminus of the gp64 derived from BmNPV to construct the recombinant plasmid pFB-gp64-Au,which was then transposed to shuttle vector Bacmid by Bac-to-Bac system.The recombinant AcMNPV baculovirus was harvested after transfection of the recombinant Bacmid into the Sf9 insect cells and its binding with colloid gold prepared by NaHB reduction method was primarily studied.The results showed that recombinant Bacmid was successfully constructed and envelope-modified baculovirus with gold-specific binding peptide was harvested.Au-bp mediated virus-nanoparticle complex was visualized by transmission electron microscopy （TEM） analysis.The study will be helpful to the research of fabricating bio-inorganic compound system and the application of nano materials in biological field.