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利用SSR标记揭示中国粳稻地方品种遗传多样性
  • ISSN号:0578-1752
  • 期刊名称:《中国农业科学》
  • 时间:0
  • 分类:S635.1[农业科学—蔬菜学;农业科学—园艺学]
  • 作者机构:[1]中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程/农业部作物种质资源利用重点开放实验室,北京100081
  • 相关基金:国家重点基础研究发展计划(“973”计划)项目(2010CB125904.5)、作物种质资源保护项日(NB2010.2130135.25-01)、围家“十一五”科技支撑计划项目(2006BAD13801)
中文摘要:

【目的】通过中国粳稻地方品种的遗传多样性分析,阐明各省份粳稻地方品种的遗传结构及其亲缘关系,为中国粳稻杂交育种的亲本选配提供科学依据。【方法】利用43对SSR多态性标记,对原产于中国17个省(市、自治区)的187份粳稻地方品种进行等位基因多样性、遗传结构和聚类分析。【结果】共检测到等位基因351个,每个位点等位基因变幅为2-21,平均每个位点等位基因数为8.2;基因多样性指数变异范围为0.117-0.908,平均为0.550;多态信息含量(PIC)变异范围为0.114-0.902,平均为0.523。在RM72、RM241、RM219、RM412和RM232等位点所检测到的遗传多样性参数值较大。西南、华南、华中粳稻地方品种的遗传多样性明显高于华北和东北地区;云南省粳稻地方品种遗传多样性最丰富,天津和吉林的粳稻地方品种的遗传多样性相对较低。基于Nei’S遗传距离的系统聚类把供试材料分为8个小类群,南方粳稻地方品种主要聚类于第i、ii、iii、iv和V等5个小类群,北方粳稻地方品种主要聚类于第vi、vi和砌等3个小类群。基于模型的群体结构分析,供试材料被分为11个亚群,有144份材料被分到相应的11个亚群,其余43份材料被分到混合亚群。【结论】南方稻区粳稻地方品种的遗传多样性明显高于北方稻区,其中西南稻区的粳稻地方品种遗传多样性最为丰富,而云南是粳稻地方品种遗传多样性最丰富的省份。北方与南方粳稻地方品种问存在较大的遗传差异,粳稻地方品种间亲缘关系与地域性存在一定的相关性,这种相关性在北方粳稻地方品种中更为突出。RM72、RM241、RM219、RM412和RM232适合应用于粳稻地方品种的遗传多样性检测。

英文摘要:

[ Objective ] The objective of this study is to analyze the genetic diversity, genetic structure and genetic relationship of japonica rice landrace from China, and to provide effective information for hybrid breeding of japonica rice. [Method] The genetic diversity, genetic structure and cluster analysis of 187 japonica rice landraces from 17 provinces (cities or municipalities ) of China were discussed using 43 pairs of polymorphic SSR markers. [ Result ] The total number of alleles detected from all tested 187 accessions was 351, and the alleles per locus ranged from 2 to 21 with the mean value of 8.2. The Nei's gene diversity indices varied from 0.117 to 0.908 with an average of 0.550. The polymorphism information content (PIC) was changed from 0.114 to 0.902 with the average of 0.523. RM72, RM241, RM219, RM412 and RM232 showed higher genetic diversity than the others. The gene diversity values (He) of japonica rice landrace from southwestern, south and central China were higher than that of north and northeastern China. The He of japonica rice landrace from Yunnan was highest compared to the other provinces, while the lowestwere Tianjin and Jilin. The dendrogram of japonica rice landrace was constructed based on Nei's genetic distance, showed that all rice varieties were subdivided into 8 clusters. The japonica rice landrace of south China contained i, ii, iii, iv and v clusters, the north China consisted of vi, vii and viii clusters. The genetic structure analysis was performed based on model, the japonica rice landrace was divided into 11 subgroups including 144 individuals and a mixed group comprising of 43 individuals. [ Conclusion ] Compared to north rice region, the south rice region showed significantly higher genetic diversity, and genetic diversity in Southwestern China of rice cropping region was the most among the 5 regions, and the center of origin of Chinese japonica rice landrace is probably Yunnan province. The japonica rice landrace from north and south was largely divergent, a

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期刊信息
  • 《中国农业科学》
  • 中国科技核心期刊
  • 主管单位:中华人民共和国农业部
  • 主办单位:中国农业科学院 中国农学会
  • 主编:万建民
  • 地址:北京中关村南大街12号中国农业科学院图书馆楼4101-4103室
  • 邮编:100081
  • 邮箱:zgnykx@caas.cn
  • 电话:010-82109808 82106279
  • 国际标准刊号:ISSN:0578-1752
  • 国内统一刊号:ISSN:11-1328/S
  • 邮发代号:2-138
  • 获奖情况:
  • 中国期刊方阵“双高”期刊,第三届中国出版政府奖提名奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),英国食品科技文摘,中国北大核心期刊(2000版)
  • 被引量:85620