中文摘要：

目的：比较当归不同炮制品挥发油对脂多糖（Lipopolysaccharide,LPS）致大鼠急性炎症的干预作用。方法：通过腹腔注射LPS（100μg/kg）建立大鼠急性炎症模型,通过血常规和血清炎性介质及细胞因子检测,结合肺脏和肝脏组织病理学观察,评价酒当归挥发油（volatile oils from parching AS with wine,J-VOAS）、当归炭挥发油（volatile oils from charred AS,C-VOAS）、生当归挥发油（volatile oils from raw AS,S-VOAS）的抗炎活性。结果：与正常对照组相比,模型组白细胞计数（white blood cell count,WBC）、中性粒细胞百分比（neutrophil percentage,NE）和血小板计数（platelet Count,PLT）显著升高（P〈0.05）,淋巴细胞百分比（lymphocytes percentage,LY）显著降低（P〈0.05）;模型组血清IL-1β、IL-6、NO、TNF-α含量极显著升高（P〈0.001）,IL-10含量极显著降低（P〈0.01）;经J-VOAS、CVOAS、S-VOAS干预后上述血常规指标和血清炎性因介质及细胞因子均显著回调（P〈0.05,P〈0.01,P〈0.001）。组织病理学观察发现,模型组大鼠肺泡扩张,肺泡及间质中大量炎性细胞浸润,肝小叶弥散性坏死,肝索排列紊乱,部分肝细胞发生颗粒变性和水肿;经J-VOAS干预后,其病理变化明显改善。结论：当归不同炮制品挥发油对LPS所致大鼠急性炎症模型均有干预作用,其中J-VOAS干预作用最好,其次为C-VOAS和S-VOAS。

英文摘要：

Objective： To compare the intervention effects of volatile oils from different preparations of Angelica sinensis root on acute inflammation induced by lipopolysaccharide in rats. Methods： Acute inflammation model was induced by intraperitoneal injection of lipopolysaccharide（ 100 μg/kg） in rats. Blood and serum inflammatory mediators and cytokines were detected,combining with the pathological histological observation of lung and liver to evaluate the anti-inflammatory activities of volatile oils from parching Angelica sinensis root with wine（ J-VOAS）,volatile oils from charred Angelica sinensis root（ C-VOAS） and Angelica sinensis root（ S-VOAS）. Results：Compared with control group,the WBC count,the percentage of NE and PLT count in acute inflammation model group significantly increased（ P〈0. 05）,and the percentage of LY significantly decreased（ P〈0. 05）; the content of IL-1β,IL-6,NO and TNF-α significantly increased（ P〈0. 001） and content of IL-10 significantly decreased（ P〈0. 05） in model group; after J-VOAS,C-VOAS and S-VOAS intervention,the blood routine index and serum inflammatory mediators and cytokines significantly reversed（ P〈0. 05）. The pathological histological study showed that expanded alveoli,massive inflammatory cells infiltration in alveoli and pulmonary interstitium,the liver leaflets diffuse necrosis,hepatic cord derangement,and some of the liver cells degeneration and edema in model group; after J-VOAS intervention,their pathological changes significantly reduced. Conclusion： All volatile oils from different preparations of Angelica sinensis root had intervention on acute inflammation induced by LPS. And J-VOAS had the best effect,followed by C-VOAS and S-VOAS.