中文摘要：

目的探讨染料木黄酮对1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）制备的帕金森病（PD）模型小鼠多巴胺能神经元的保护作用。方法将C57BL6雄性小鼠随机分为正常对照组、MPTP模型组及染料木黄酮预处理组。用逆转录-聚合酶链式反应（RT-PCR）技术检测黑质酪氨酸羟化酶（TH）及多巴胺转运体（DAT）基因的表达水平。应用高效液相色谱法检测纹状体（Str）内DA及其代谢物二羟基苯乙酸（DOPAC）含量。结果MPTP模型组黑质TH和DAT基因的表达较正常对照组明显降低,染料木黄酮预处理组可逆转上述改变（F=20.31、13.19,P〈0.01）。MPTP模型组Str内DA及其代谢产物DOPAC的含量较对照组明显减少,应用染料木黄酮后可逆转上述改变（F=63.58、16.12,P〈0.01）。结论染料木黄酮对MPTP制备的PD模型小鼠黑质纹状体DA能神经元有明显的保护作用。

英文摘要：

Objective To investigate the protective effect of genistein on doparminergic neurons. Methods C57BL6 mice were randomly divided into three groups, control group （DMSO, ip）, MPTP group （15 mg/kg, ip） and genistein-pretreatment （10 mg/kg, ip） group. The changes of tyrosine hydroxylase （TH） and dopamine transport （DAT） gene expression in substantia nigra （SN） were detected by using reverse transeription-polymerase chain reaction （RT-PCR）. The contents of DA and its metabolites dihydroxy-phenylacetic acid （DOPAC） were determined by high performance liquid chromatography-electrical chemical detection （HPLC-ECD）. Results Genistein pretreatment could prevent the MPTP-indueed decrease of TH and DAT gene ex- pression （F=20.31,13.19;P〈0.01）. MPTP significantly decreased the levels of dopamine （DA） and its metabolites dihydroxy- phenylacetic acid （DOPAC） in the striatum, which could be restored by genistein pretreatment （F=63. 58,16. 12; P〈0.01）. Conclusion Genistein has neuroproteetive effects on dopaminergie neurons against MPTP-indueed mouse model of PD.