目的探讨人参皂苷Rg1对6-羟基多巴胺(6-OHDA)所致MES23.5神经细胞损伤的保护作用。方法MES23.5细胞常规培养,观察人参皂苷Rg1预处理对6-OHDA毒性作用的影响,MTT法观察细胞存活率,实时荧光半定量反转录聚合酶链反应(real time RT-PCR)观察酪氨酸羟化酶(TH)和Bc1-2基因的表达情况。结果6-OHDA可剂量依赖性地损伤MES23.5细胞(F=71.24,P〈0.01),人参皂苷Rg1预处理可对抗6-OHDA的毒性作用(F=14.63,P〈0.01);6-OHDA可明显降低TH和Bc1-2基因的表达,人参皂苷Rg1预处理可明显逆转上述改变(F=9.80、15.34,P〈0.01)。结论人参皂苷Rg1可明显对抗6-0HDA对MES23.5神经细胞的损伤,其作用机制可能与抗凋亡有关。
Objective To study the protective effect of ginsenoside Rg1 against 6-OHDA-induced neurotoxicity in MES23.5 ceils. Methods MES23.5 cells were routinely cultured and pretreated with ginsenoside Rgl to determine the protective effect of Rgl against 6-OHDA-induced neurotoxicity. The cell viability was observed by MTT method. The gene expressions of TH and Bc1-2 were detected by real time RT-PCR method. Results 6-OHDA induced cell death in a dose-dependent manner. Pretreatment with ginsenoside Rg1 had neuroprotective effects on cell viability against 6-OHDA-induced toxicity. 6-OHDA decreased the gene expressions of TH and Bc1-2. These effects could be reversed by Rg1 pretreatment. Conclusion Ginsenoside Rg1 has neuroprotective effects against 6-OHDA-induced neurotoxicity in MES23.5 cells, its mechansim may be related to its antiapoptosis effects.