中文摘要：

目的探讨人参皂苷Rg1对抗6-羟基多巴胺（6-OHDA）毒性作用的信号通路。方法MES23.5细胞常规培养,免疫印迹法观察人参皂苷Rg1预处理对6-OHDA诱导的磷酸化蛋白激酶B（Akt）表达的影响,MTT法观察细胞的存活率。结果6-OHDA可时间依赖性地降低MES23.5细胞磷酸化Akt的表达（F=24.51,P〈0.01）,人参皂苷Rg1预处理可明显增强磷酸化Akt的表达（t=471.60,P〈0.01）;人参皂苷Rg1预处理可明显降低6-OHDA对MES23.5细胞的损伤作用,此作用可以被磷脂酰肌醇3激酶（PI3K）特异性抑制剂LY294002所阻断（F=25.12,P〈0.01）。结论人参皂苷Rg1通过激活PI3K/Akt信号通路对抗6-OHDA对MES23.5神经细胞的毒性作用。

英文摘要：

Objective To study the signaling pathway involved in the protective effect of ginsenoside Rg1 against 6-OHDA-induced toxicity. Methods MES23.5 cells were routinely cultured and the effect of ginsenoside Rg1 against the 6-OHDA induced Akt phosphorylation was detected by western blot method, and the cell survival observed by MTT method. Results 6- OHDA inhibited the Akt phosphorylation in a time dependent manner in MES23.5 cells （F= 24.51, P〈0.01）. Pretreatment with ginsenoside Rg1 could increase the Akt phosphorylation （F=12.37,P〈0.01）. Pretreatment with ginsenoside Rg1 could decrease the 6-OHDA induced toxicity in MES23.5 cells and these effects could be completely blocked by the PI3K inhibitor LY294002 （t= 471.60,P〈0.01）. Conclusion Ginsenoside Rg1 protects against 6-OHDA induced neurotoxicity via the activation of the PI3K/ Akt signaling pathway in MES23.5 cells.