中文摘要：

目的：探讨人参皂甙Rg1对6-羟基多巴（6-OHDA）制备的去卵巢（OVX）帕金森病（PD）模型大鼠黑质（SN）多巴胺能神经元的保护作用及其可能机制。方法：应用6-OHDA制备的OVX PD模型大鼠,侧脑室给予Rg1或雌激素。免疫组织化学染色酪氨酸羟化酶（TH）阳性神经元和Bcl-2蛋白。Perls’铁染色检测SN铁含量。结果：①Rg1或雌激素可抑制阿朴吗啡诱导的PD大鼠旋转行为;②在损毁侧SN,Rg1或雌激素用药组TH阳性神经元数量较6-OHDA组显著增多;③6-OHDA组损毁侧SN内铁含量较健侧明显升高,应用Rg1或雌激素后,SN铁含量较模型组明显减少;④与6-OHDA模型组相比,Rg1及雌激素均可增加损毁侧大鼠SN内Bcl-2蛋白表达。结论：人参皂甙Rg1具有类雌激素样作用,对OVX PD模型大鼠黑质DA能神经元有明显的保护作用,其作用机制可能与降低铁负载和抗凋亡有关。

英文摘要：

Aim： To investigate the neuroprotective effect of ginsenoside Rgl on dopaminergic neurons of substantia nigra in ovariectomized rat model of Parkinson＇ s disease and the possible mechanisms. Methods： Wistar female rats were ovariectomized and treated with vehicle, ginsenoside Rgl or 17-13 estradiol intracerebroventricularly in the 6-OHDA induced rat model of Parkinson＇s disease. Immuno-histochcmistry was used to detect the tyrosine hydroxylase （TH） immunoreactive neurons and the protein expression of Bcl-2. Perls＇ iron staining was used to determine the changes of iron in substantia nigra （SN）. Results： （1）Rgl or 17-0 estradiol treatment could ameliorate the rat＇s rotational behavior induced by apomorphine. （2）Rgl or 17-β estradiol treatment could increase TH immunoreactive neurons in the injured side of SN compared to the 6-OHDA group. （3）Iron staining in the injured side of SN was significantly increased comparing with the contralateral side in the 6-OHDA group. Rgl or 17-β estracliol treatment could reverse the increase of iron staining. （4）Both Rgl and 17-β estradiol treatment could increase Bcl-2 protein expression in the injured side of SN compared to the 6- OHDA group. Conclusion： Ginsenoside Rgl has estrogen-like activities and has neuroprotective effects on the dopaminergic neurons in the 6-OHDA induced ovariectomyzed（OVX） rat model of parkinson＇ sldisease（PD）. This effect may be attributed to attenuating iron overload and anti-apoptosis.