中文摘要：

目的 研究染料木素（genistein）对6-OHDA制备的帕金森病（PD）模型去卵巢（OVX）大鼠黑质（SN）多巴胺能神经元、铁含量及Bcl-2蛋白表达的影响.方法 应用6-OHDA制备的OVX PD模型大鼠,侧脑室给予genistein或（17-β-estradiol）17-β雌二醇.免疫组织化学染色酪氨酸羟化酶（TH）阳性神经元和Bcl-2蛋白.Perls铁染色检测SN铁含量.结果 Genistein和17-β-estradiol可明显减少6-OHDA制备的PD模型大鼠单侧旋转行为（P＜0.01）.在损毁侧SN,genistein和17-β-estradiol能促进TH阳性神经元数量显著增多（P＜0.01）,Bcl-2蛋白表达明显增加（P＜0.01）,铁染色阳性细胞数量明显减少（P＜0.01）.结论 genistein具有类雌激素样作用,通过降低SN铁含量及增加Bcl-2蛋白的表达,发挥其对6-OHDA所致PD模型大鼠SN多巴胺神经元的保护作用.

英文摘要：

Objective To investigate the effect of genistein on dopaminergie neurons, iron contents and Bcl-2 protein expression of substantia nigra （SN） in 6-OHDA-indueed PD model of ovariectomized female rats. Methods Wistar female rats were ovariectomized and treated with, genistein or 17-β-estradiol intraeerebroventrieularly for 4 weeks after unilateral injection of 6-OHDA into the medial forebrain bundle （MFB）. Immuohistochemistry was used to detect the positive neurons of tyrosine hydroxylase （TH） and protein expression of Bcl-2. Perls iron staining was used to determine the changes of iron contents in substantia nigra （SN）. Ressults Both genistein and 17-β-estradiol could ameliorate the rat＇ s rotational behavior induced by apomorphine in PD model rats （P〈 0.01）. Both genistein and 17-β-estradiol could prevent the 6-OHDA-indueed decrease of TH immunoreaetive neurons and Bcl- 2 protein expression in the ipsilateral SN in PD model rats （P 〈 0.01 ）, and reverse the increase of iron staining （P 〈 0.01）. Condusion Genistein has estrogen-like activities and neuroprotective effects on the dopaminergie neurons in the 6-OHDA induced rat model of PD through decreasing iron content and increasing Bcl-2 protein expression.