采用FISH技术对2008年冬、夏两季太湖梅梁湾水体中细菌优势种群的丰度进行了对比分析.实验中,选取荧光素标记的特异寡核苷酸探针,与太湖梅粱湾水体中的四类细菌优势种群α-变形菌(α-proteobacteria),β-变形菌(β-proteobacteria),γ-变形菌(γ-proteobacteria)和拟杆菌(Bacteroidetes)进行原位杂交.结果表明:①所测定的四类细菌优势种群占总细菌的比例在冬季均显著高于夏季(P〈0.01);②水体中α-proteobacteria的丰度随TP浓度的增加而显著增加(R^2=0.860,P〈0.01);③随着Chl.a浓度的增加,水体中细菌的丰度呈上升的趋势,尤其是其中的y-proteobacteria,其增大趋势更加显著(R^2=0.857,P〈0.01);④细菌种群的丰度受水体中PO4^3-浓度的影响,尤其是对Bacteroidetes的影响更加显著(R^2=0.733,P〈0.05).
FISH was used to analyze the abundance of dominated bacteria population in Meiliang Bay of Lake Taihu in winter and summer in 2008. The four dominated bacteria population ( α-proteobacteria, β-proteobacteria, T-proteobacteria, Bacteroidetes) were analyzed by in situ hybridization with specific fluorescently (FITC) labeled rRNA-targeted oligonucleotide probes in the test. The results indicated : (1) The proportion of each of the four dominated species in the total number of bacteria was all significantly higher in winter than that in summer( P 〈0. 01 ) ; (2) the abundance of α-proteobacteria rose notably with the increase of TP concentration ( R^2 = 0. 860, P 〈 0. 01 ) ; (3) there was ascending trend in the abundance of bacteria along with the increase of Chl. a concentration, especially that of γ-proteobacteria was more remarkable than the others ( R2 = 0. 857, P 〈 0. 01 ) ; (4) the abundance of bacteria population, especially for Bacteroidetes, was also influenced by PO4^3 - concentration (R^2 = 0. 733, P 〈 0. 05 ).