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中文摘要：
目的 探讨磷脂酰肌醇-3-激酶（PI3K）信号通路在骨髓间充质干细胞（BMSCs）向胶质瘤定向迁移中是否发挥作用.方法 骨髓间充质干细胞分离自6周龄的SD大鼠,采用全骨髓贴壁法原代培养,然后利用流式细胞技术检测BMSCs的表面标志物.利用Transwell小室构建骨髓间充质干细胞体外迁移模型,利用大鼠C6及人U87胶质瘤细胞条件培养基诱导BMSCs的趋化迁移,观察体外BMSCs向胶质瘤的迁移情况.对照组为24孔板下室加入不含细胞的空白无血清培养基,实验组为下室内分别加入0.5 mL的C6或U87胶质瘤细胞条件培养基.然后在Transwell上室加入抑制浓度为10 μmol/L的PI3K信号通路抑制剂LY294002,观察BMSCs迁移能力的变化情况.结果 分离培养得到的细胞具有间质干细胞典型的细胞形态,不表达CD34、CD45,阳性表达CD90及CD73.Transwell体外迁移模型显示C6及U87胶质瘤条件培养基强烈吸引了BMSCs的迁移.加入PI3K信号通路抑制剂LY294002后,C6及U87条件培养基诱导的迁移到半透膜下表面的BMSCs细胞数量均显著减少,BMSCs向胶质瘤的迁移受到抑制.结论 在骨髓间充质干细胞向胶质瘤的定向迁移过程中,PI3K信号通路发挥作用.
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