以拟南芥Columbia(Col.)生态型和Fibrillins突变体(fib8和fib1a)为研究对象,对镉胁迫条件下植株的萌发、根长生长以及植物体内H2O2和MDA含量及CAT活性的变化进行了测定.本研究发现,30μmol/LCd2+浓度胁迫条件下,Col、fib8和fib1a的萌发未受到抑制,但根长生长都受到了抑制,fib8和fib1a的抑制程度比Col更为明显,表明突变体比野生型对镉胁迫更敏感.进一步测定镉胁迫前后Col、fib8和fib1a体内的H2O2和MDA含量及CAT活性的变化,结果显示Col、fib8和fib1a体内的H2O2含量增加量分别为处理前的1倍、10倍、8倍,Col、fib8和fib1a体内的MDA含量增加量分别为处理之前的2倍、14倍、12倍,Col、fib8和fib1a体内的CAT活性下降程度相似约为20%.由实验结果推测Fibrillins可能通过影响植物体内H2O2和MDA含量的变化参与植物响应重金属镉胁迫.
The effects of Cd2+ on germination rate, root length and H2O2 content ,MDA content,CAT activity in Arabidopsis thaliana (Col.) and Fibrillins mutant(fib8 and fibla were used in our reasearch) seedlings were studied. The germination rate of Col,fib8 and fibla almost have no inhibition under 30 /2mol/L Cd2+. Root growth experiment showed that fib8 and fibla were more sensitive than Col on cadmium treatment because the root length of fib8 and fibla were inhibited clearly. Moreover, the influence of H2O2content,MDA content and CAT activity. Caused by Cd2+ were determined. It was found that H2O2in Col,fib8 and fibla up 1 times,10 times,8 times and MDA content in Col,fib8 and fibla up 2 times,14 times,12 times from pretreatment. Meanwhile, CAT activity in Col,fib8 and fibla seedlings down slightly by 20%. So it was concluded that Fibrillin are involved in plant response to heavy metal stress by affect H2O2 and MDA in plants.