中文摘要：

以At豫1作为诱饵蛋白利用泛素分裂系统在拟南芥cDNA文库中筛选与拟南芥AtTRl基因相互作用的蛋白质．运用营养缺陷型培养基和X-Gal显色、PCR扩增及酶切分析等实验排除假阳性，并对阳性克隆进行序列测定和比对．通过泛素分裂系统筛选到可靠的At—TR1相互作用蛋白质，测序比对后，选取HMA与AtTRl通过GSTpull-down进行进一步的验证，结果证明它们确实存在相互作用，这对进一步研究At豫1及其相互作用蛋白质在植物耐热中的作用和耐热分子机制奠定了基础．

英文摘要：

Using the new type of two-hybrid system-DUAL membrane system to screen proteins which interact with AtTR1. AtTR1 was used as a bait protein to screen the Arabidopsis thaliana cDNA librar y. Selective media, X-Gal,PCR amplification and restriction enzyme analysis were used to exclude the false positive. Then the positive clones were sequenced and blast. Using the DUAL membrane system find out the potential interacting partners of AtTR1. After sequencing and blasting,one protein named HMA was selected and further confirmed interacting with AtTR1 by GST pull-down. The experimental results showed that AtTR1 can interact with HMA in vitro. The study provides the foundation for fur ther understanding of the functions and the heatresistant molecular mechanism.