中文摘要：

为了分析拟南芥脱落酸响应蛋白RING-V1基因（ABRv1）的功能，构建了高表达载体pBI121-ABRv1，经农杆菌转化，花序浸染后得到TO代转基因种子，并经卡那霉素板筛选得到转基因苗，再经过两代的筛选获取了纯舍的高表达株系ABRv1．通过DNA及RNA水平鉴定了ABRv1基因的T-DNA插入突变体abrv1．对突变体、高表达转基因株系及野生型进行ABA诱导处理，突变体的萌发率不足109／6，野生型萌发率为40％，而过表达株系萌发率为67％；突变体株系气孔几乎全部关闭，过表达株系气孔关闭不明显，大小是突变体株系的2～3倍．结果表明ABR可1基因在拟南芥的ABA信号应答响应中可能起负调控作用．

英文摘要：

In an effort to analyze the function of ABscisic acid responsive RING-v protein 1 （ABRv1） in Arabi- dopsis thaliana, this paper performed abscisic acid （ABA） stress experiments on the wild-type, the ARBvl- overexpressing lines and the T-DNA insertion mutants abrv1. The overexpressing lines were acquired through transfection by Agrobacterium with ABRvl embedded plasmids （pBI121-ABRv1）. The mutants obtained were identified by DNA and RNA analysis. With ABA treatment, the mutants germination rate was less than 10%, while the wild-type was 40% and the overexpressing lines was 67%. Little stomatal closure was observed in the overexpresssing lines whereas obvious closure was discovered in the mutants. Stoma size of overexpressing lines was twice or triple of the mutants. The wild-type showed a closure status in between. These phenotypic analysis revealed that ABRv1 might participate in the plant response to ABA as a negative regulator.