中文摘要：

目的运用代谢组学方法研究慢性阻塞性肺疾病（COPD）稳定期肺气虚证及其中药干预的尿液代谢组学特征，探索其可能存在的生物标记物。方法选择40例COPD稳定期肺气虚证患者（肺气虚证组），给予六味补气胶囊进行干预，并设健康对照组37例。分别对健康对照组及肺气虚证组药物干预前后进行肺功能检查；采用高效液相色谱一二级质谱联用仪检测尿液代谢物，利用主成分分析（PCA）和偏最小二乘法（PLS）对数据进行统计分析，比较2组尿液代谢组学原始谱图和数据。结果与健康对照组比较，肺气虚证组治疗前肺功能明显下降（P〈0．05），治疗30d后仍低于健康对照组。肺气虚证组治疗前的代谢谱与健康对照组明显不同，但治疗后有向健康对照组回归的趋势。发现13种COPD肺气虚证可能的疾病标记物。结论运用尿液代谢组学方法可以发现COPD稳定期肺气虚证患者存在代谢谱改变，并发现其潜在的疾病标记物。对COPD稳定期肺气虚证患者进行中医药干预，其代谢谱具有积极的变化。

英文摘要：

Objective To study the characteristics of urine metabolomics of lung qi deficiency syndrome of stable-phase chronic obstructive pulmonary disease （COPD） and intervention of Chinese medicinal, and explore possible biomarkers. Methods The patients （n = 40） with lung qi deficiency syndrome of stable-phase COPD （lung qi deficiency group） were intervened with Liuwei Buqi Capsules, and a control group （ n = 37 ） was set up. The two groups were given the examination of lung function before and after the intervention. The urine metabolites were detected by using HPLC-MS/MS, and data was statistically analyzed by applying principle component analysis （PCA） and partial least squares （PLS）. The original spectrum and data of urine metabolomics were compared between two groups. Results Compared with control group, lung function decreased significantly in lung qi deficiency group before treatment （P 〈0.05 ）, and still lower after treatment for 30 days. The metabolic spectrum was significantly different in lung qi deficiency group compared with control group before treatment, but showed regressivetrend to control group after treatment. There were 13 possible biomarkers of lung qi deficiency syndrome of COPD found. Conclusion The changes of metabolic spectrum and potential disease biomarkers can be found in patients with lung qi deficiency syndrome of stable-phase COPD The intervention of Chinese medicinal will bring the positive changes in metabolic spectrum.