中文摘要：

人神经细胞SH—SY5Y常规法培养至长满细胞瓶（120cm^2）后均分为A～I 9个小细胞瓶（30cm^2），37℃培养15h后，B～D组在42℃条件下分别进行0．5、1和2h的持续热应激处理，而E～I组于42℃热应激处理0．5h后，37℃分别恢复1、3、8、12、24h。持续热应激阶段，朊蛋白（PrP）和热休克蛋白（HSP）90 mRNA水平显著提高（P〈0．01），应激2h时，PrP和HSP90 mRNA水平分别约为对照组（A组）的2．5和5倍；在应激后恢复初期（1～3h），PrP和HSP90 mRNA水平也显著升高（P〈0．01），其中恢复到3h时，PrP和HSP90 mRNA水平分别为对照组的9和7倍；当恢复到12～24h时，HSP90和PrP mRNA的转录水平与对照组差异不显著。结果表明热应激可以提高HSP90和PrP mRNA的转录水平。应激时PrPmRNA转录水平的提高暗示着PrP在细胞应激中起着一定的生物作用。

英文摘要：

Cells were maintained at 37℃ in a humidified 50% CO2/air incubator. Once the cells became confluent in the large flask （120 cm^2 in size）, they were split equally and distributed into nine （A-I） smaller flasks（30 cm^2 in size）. The cells in the smaller flasks were kept at 37 ℃ in the incubator for 15 h prior to heat-shock treatment, at each time, B-D flasks were removed to 42℃ incubator for 0. 5, 1, 2 h and E-I were recovered into the incubator at 37℃ for 1, 3, 8, 12, 24 h subsequent to heat-shock treatment at 42℃ for 0.5 h. There was an about 2.5 and 5-fold increase in PrP and HSP90 mRNA levels 2 h following heat shock at 42 ℃. In addition, a 9 and 7-fold increase in mRNA level of PrP and HsP90 was found following heat shock 0.5 h at 42 ℃ and recovery for 3 h at 37 ℃ and there was no significant difference with control following heat shock 0.5 h at 42 ℃ and recovery for 12 h and 24 h at 37 ℃. These results suggested that heat stress induced the elevation of both PrP and HSP90 mRNA. The up-regulation of prion-protein mRNA implies that PrP may play a role in cellular stress.