中文摘要：

选取12头皮特兰和24头二花脸仔猪，体重达到（20±1）kg时，随机选取6头皮特兰和12头二花脸进行2h运输，其余仔猪作为对照组。采用HE染色、免疫组织化学以及酶联免疫吸附试验技术，通过对不同品种仔猪运输前后肝脏、肾脏、胃的损伤以及Hsp47的定位、定量观察，研究运输应激与Hsp47表达之间的关系。结果表明：2h运输应激后，二花脸和皮特兰仔猪各组织的实质细胞均有不同程度的损伤；Hsp47主要分布在肝脏的中央静脉和窦状隙内皮细胞、肾小球和远曲小管上皮细胞、胃的黏膜层和黏膜下层结缔组织内；在肾脏和胃组织中，皮特兰仔猪在运输后Hsp47含量增加（P〈0．05），但二花脸仔猪的相应值未见显著差异，2个品种肝脏中Hsp47含量在运输前后的变化无差异。Hsp47在受检组织中尽管有表达增加的趋势，但2个不同品种仔猪组织中Hsp47含量升高的程度不同，说明二者对运输应激的敏感性不同，二花脸仔猪具有更强的抗应激能力。

英文摘要：

12 Pietrain （PIE） and 24 Erhualian （EHL） piglets （20 kg±1 kg） were randomly devidedinto2 （transported and control） groups. 6 PIE and 12 EHL were transported for 2 h, the rest piglets were maintained under normal conditions as the control. By means of histopathological method, immunohistochemistry and enzyme linked immunosorbent assay, the tissue damages, the localization and the quantification of Hsp47 in the same tissues such as liver, kidney, stomach were detected in different breeds of transported piglets. After 2 h transportation there were obvious degeneration in the liver, kidney and stomach of transported piglets; Hsp47 were predominantly expressed in the endothelial cells of central vein and hepatic sinusoid in liver, in the endothelial cells of renal glomeruli in kidney, in the connective tissues of the mucosa and submucosa in stomach; The expressions of Hsp47 in kidney and stomach of PIE transported piglets were significantly higher than those in the control group （P〈0.05）, but there was no obvious difference in EHL piglets. It seems there were no obvious Hsp47 changes in livers of both PIE and EHL piglets. The induction degrees of Hsp47 expression displayed different changes in the tissues of transported and control piglets between two different breeds. It revealed that there were different sensitivities to transport stress for two breeds. EHL piglets gave more advantage to anti stress.