欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
根据hsp70、hsp90和GAPDH基因mRNA序列，设计并合成3对引物，将PCR扩增的基因片段克隆到pGEM-T载体，重组质粒经筛选、鉴定，体外转录出RNA，系列稀释作为阳性标准品，用于标准曲线的绘制和样品检测，并用持家蛋白GAPDH基因对样品进行归一化，建立了一步法实时荧光定量RT-PCR技术平台，并对其扩增效率、敏感性、特异性进行了检测。结果证明，建立的一步法荧光定量RT—PCR技术可以真实地反映反应管中模板的数量，可用于猪矗驴。、hsp。和GAPDH基因mRNA水平的检测。
英文摘要：
According to the specific sequence of hsp90, hspgo and GAPDH mRNA, the primers were designed and synthesized. The fragments generated by RT-PCR were cloned into the pGEM-T vector. The positive recombinant plasmids were used to transcript RNA in vitro and the RNA were used as standard quantitative template to make the standard curve and establish fluorescence quanti- tative RT-PCR （ FQ RT-PCR） method. The efficiency, sensitivity and specificity of the RT-PCR method were tested. The results showed that the FQ RT-PCR method was external and could be used to detect the hsp70, hsp90 and GAPDH mRNA transcription of pigs.
同期刊论文项目
同项目期刊论文
Characterization of adrenal ACTH signaling pathway and steroidogenic enzymes in Erhualian and Pietra
Meat quality is associated with muscle metabolic status but not contractile myofiber type compositio
Meat quality is associated with muscle metabolic status but not myofiber type composition in prematu
Diminished expression of ACTH signaling proteins and steroidogenic limiting factors in adrenocortica
期刊信息
位置：