中文摘要：

【目的】研究长途运输应激对猪免疫器官的病理性损伤、HSPs mRNA的分布和转录水平,探讨其相关性。【方法】利用组织学、原位杂交和本实验室建立的猪HSPs mRNA一步法实时荧光定量RT-PCR技术平台等方法,研究长途运输应激对猪免疫器官的病理性损伤、HSPs mRNA的分布和转录水平的变化。【结果】在长途运输应激初期（1～2h）,淋巴结和脾脏出现急性病理性损伤变化,损伤程度较为严重;运输应激4h时,组织的病理性损伤与应激初期相似,但血管的充血程度有所减轻;淋巴结和脾脏中HSP70 mRNA的转录水平随着运输应激时间的延长一直呈现出上升的趋势,运输应激持续到10h时,HSP70 mRNA的转录水平升到最高,分别为对照组的9.59和11.46倍;HSP90 mRNA的转录水平在应激初期（1～2h）急剧升高（P〈0.01）,运输应激2h时,淋巴结和脾脏中HSP90 mRNA的转录水平分别为对照组的59.67和13.03倍,到运输应激持续到6h后,升高不再显著。HSPs mRNA在淋巴结和脾脏细胞中的定位无肉眼可见的随着应激时间的延长而发生相应的变化,但HSP70和HSP90 mRNA之间的定位存在着差异。【结论】运输应激可以导致组织损伤和HSPs mRNA转录水平的变化,但对不同家族的HSPs mRNA转录水平的影响不同;HSP90 mRNA的转录水平与组织损伤有着良好的相关性,有望成为猪运输应激的判定指标之一。

英文摘要：

【Objective】To study the relationship between the distribution,transcription level of HSPs mRNA and immunity tissue pathological lesion of transport stressed pigs. 【Method】 In situ hybridization,one-step fluorescence quantitative and histopathological methods were used to invest pathological lesion,HSPs mRNA distribution and transcription level in lymph node and spleen of transport stressed pigs. 【Result】Obvious acute histopathological changes were observed in lymph node and spleen at the beginning of transport stress （1-2 h）. The level of HSP70 mRNA transcription increased continuously from the beginning of transportation. The inductions of HSP70 mRNA transcription in lymph node and spleen of 10 h transport stressed pigs were 9.59 and 11.46 times higher than that of the un-transport stressed pigs （P〈0.01）. However,the transcription levels of HSP90 mRNA increased only at the beginning of transport stress,59.67 and 13.03 times higher than that of the un-transport stressed pigs at 2 h transport stressed pigs in lymph node and spleen,respectively. The location of HSP70 and HSP90 mRNA did not change with the transport stress. 【Conclusion】Transport stress can affect the transcription of HSPs mRNA,and the effect is different between HSPs families.Furthermore,there is a good relationship between the concentration of HSP90 mRNA and pathological lesion of lymph node and spleen,and HSP90 mRNA might be a hallmark to determine stress.