中文摘要：

目的：探讨腺苷A2A受体、多巴胺D2受体及纹状体-苍白球通路在运动疲劳调控中的作用。方法：Wistar雄性健康大鼠30只,随机分为3组：运动对照组（CG）、腺苷A2A受体拮抗剂干预组和多巴胺D2受体激动剂干预组,每组10只。3组大鼠从纹状体分别微量注射人工脑脊液、特异性腺苷A2AR拮抗剂SCH58261和D2DR激动剂喹吡罗,采用金属微电极分别记录1次力竭运动过程中大鼠同侧苍白球局部场电位的动态变化,结合运动能力进行观察并记录力竭运动时间。结果：苍白球神经元电活动在力竭运动不同阶段出现明显变化特征,相比安静状态,力竭即刻神经元兴奋性显著下降,β波的PSD值增加（P〈0.05）,γ波PSD值显著下降（P〈0.05）,功率谱重心频率降至最低（P〈0.05）。A2AR拮抗剂和D2DR激动剂干预能够改变相应波段的振幅,在运动开始75 min、力竭即刻与恢复期,均显著高于CG（P〈0.05）,延缓重心频率的下降和大鼠跑台运动至力竭的时间。结论：1次力竭运动过程中大鼠苍白球神经元局部场电位随着运动强度和时间的增加而呈现阶段性变化特征,主要体现为出现明显的振荡活动（β波和γ波振荡）,且β波和γ波之间的转换和功率的变化与运动状态有关;苍白球神经元的兴奋性下降可能是导致运动疲劳产生的中枢机制之一,证明苍白球参与运动疲劳的中枢调控;SCH58261和喹吡罗经纹状体微注射后,可以通过各自受体抑制力竭时D2-MSNs的过度兴奋,调节β波和γ波频段的振幅,从而避免苍白球神经元兴奋性下降过快,降低苍白球通过皮层-基底神经节环路对皮层的抑制作用,延缓疲劳发生。

英文摘要：

Objective： This study aimed at exploring the role of adenosine A2A receptors （A2, R） , dopamine 1）2 receptor （D2DR） and striato-pallidal pathway in the regulation of exercise fatigue. Methods： Thirty male healthy Wistar rats were randomly divided into 3 groups： exereise control group （CG, n = 10） , A2A R antagonists group （AG, n = 10） and DeDR agonist group （DG, n = 10）. Rats of three groups were infused artificial cerebrospinal fluid, A2AR antagonist SCH58261 and D2DR agonist quinpirole through corpus striatum respectively. The dynamic change of pallidal local flied potentials in an exhaustive exercise process was recorded by metal mieroelectrode, and the exhaustive exercise duration was recorded. Results ： The electrical activity of pallidus neurons had obvious change in different stages of exhaustive exercise. Compared with quiet state, the neuronal excitability decreased, PSD of βwave increased （ P 〈 0. 05 ）, PSD of 3＇ wave decreased （ P 〈 0.05 ）, and the gravity frequency of PSD decreased to the lowest （ P 〈 0.05 ） immediately after exhaustion. The A2A R antagonists and D2 DR agonists changed the amplitude of wave band, the amplitude of those groups was higher than that of CG at 75 min exercise, immediately after exhaustion and recovery period （P 〈 0.05 ） ; and A2A R antagonists and D2 DR agonists slowed down the decrease of gravity frequency, and extended the exhausted exercise duration. Conclusions ： During an ex- haustive exercise, the pallidal neurons local filed potentials of rats show characteristics of phase change with the increase of exercise intensity and duration, mainly in obvious oscillatory activity （18 and ~ waves） ; the conversion and power change of and 4! waves are related to exercise state; the decrease of globns pallidus neurons electricity may be one of the central mechanisms leading to exercise-induced fatigue, which demonstrated that globus pallidus is involved in central regulation of exercise fatigue; SCH58261 can inhibi