中文摘要：

目的：通过观察一次性力竭运动过程中大鼠苍白球内侧部（GPi）神经递质谷氨酸（Glu）和γ-氨基丁酸（GABA）及相关受体表达水平的动态变化,探讨力竭运动过程中苍白球内侧部神经元电活动变化的可能机制。方法：选取雄性Wistar大鼠为实验对象,采用微透析-高效液相色谱联用技术,观察一次性力竭运动过程中大鼠苍白球内侧部神经元胞外Glu和GABA水平动态变化;采用免疫荧光双标技术,检测安静、力竭时和恢复90 min时谷氨酸离子型2B受体（NR2B）和γ-氨基丁酸A型受体α1亚基（GABAAα-1）表达水平。结果：自主运动期大鼠苍白球内侧部神经元胞外Glu/GABA比值均显著下降（P〈0.05）,而力竭即刻其胞外Glu/GABA比值则显著升高（P〈0.01）。与安静状态相比,苍白球内侧部在恢复90 min时NR2B受体阳性细胞数量显著增加（P〈0.05）,力竭即刻和恢复90 min时GABAAα-1受体阳性细胞数量显著减少（P〈0.05,P〈0.01）。结论：力竭运动过程中大鼠苍白球内侧部Glu、GABA浓度及NR2B、GABAAα-1受体蛋白表达的改变是导致该核团神经元兴奋性变化的原因之一。

英文摘要：

Objective：The purpose of this study was to reveal the possible mechanism of the neurons activity change in globus pallidus（GPi） during exhaustive exercise by observing the dynamic changes of glutamate（Glu）,γ-aminobutyric acid（GABA） and their receptors in internal segment in rats ＇ GPi during exhaustive exercise.Methods：Male Wistar rats were selected in this study.The expressions of the Glu and GABA in GPi during an exhaustive exercise were observed by microdialysis-high performance liquid chromatogram techniques.The expressions of NR2 B receptor and GABAAα-1 receptor were detected at rest,during exercise,90 min after exercise,by double-lable immunofluorescence technique.Results：Compared with rest state,the Glu / GABA ratio of GPi significantly decreased during the auto-exercise period（P 0.05）,while it was significantly increased at exhaustion time point（P 0.01）.The number of NR2 B positive cells in GPi was increased significantly at 90 min after exhaustive exercise compared with rest state（P 0.05）,but number of GABAAα-1 positive cells was decreased significantly both at exhaustion time point（P 0.05） and 90 min after exhaustion（P 0.01）.Conclusion：The change of expressions of Glu,GABA,NR2 B receptor and GABAAα-1 receptor in rats＇ GPi during exhaustive exercise is a reason for neurons activity change.