中文摘要：

目的：干预基底神经节直接通路和间接通路,观察运动疲劳后苍白球内侧部（GPi）和黑质网状部（SNr）神经元电活动变化,探讨GPi/SNr在基底神经节信息整合中的作用。方法：选取大鼠建立跑台运动疲劳模型,采用不同频率的电刺激干预间接通路,微量注射KA、SCH23390干预直接通路,记录运动疲劳前后GPi/SNr神经元电活动。结果：刺激丘脑底核引起GPi/SNr兴奋性反应的放电频率在一定范围内随刺激频率增大而增大;疲劳组GPi/SNr神经元对变频刺激的反应较安静组增强;增加刺激频率时,GPi/SNr神经元兴奋性反应放电频率减小。特定刺激频率作用下,疲劳组GPi/SNr神经元兴奋性反应比例和放电频率显著高于安静组（P〈0.05）;疲劳组SNr兴奋性、抑制性反应频率均比GPi高。分别微量注射KA非诺多泮后,SNr疲劳组的抑制性反应比例显著高于安静组（P〈0.05）;微量注射SCH23390后,疲劳组兴奋性反应比例显著高于安静组（P〈0.05）。结论：GPi/SNr作为基底神经节的输出核团,在整合直接、间接通路信息的过程中存在不同的信号处理方式。运动疲劳后,干预间接通路时引起GPi/SNr神经元反应的高频刺激阈值增加,减少STN对GPi/SNr的兴奋性传入可能成为干预运动疲劳间接通路的一种手段。

英文摘要：

Objective： In order to reveal the integration role of GPi / SNr in central fatigue,this research recorded the neuron activity after different interventions on direct and indirect pathways. Methods： Exercise induced fatigue model was established in rats,and electrical stimulations with different frequencies were used to disturb direct pathway while microinjections with KA,SCH23390 were used to stimulate indirect pathway,and the firing activity of GPi / SNr was recorded before and after fatigue. Results： GPi / SNr evoked discharge increased by degrees within a certain range when the stimulus frequency increased; reaction of GPi / SNr neurons to different frequency stimulations in fatigue group was higher than in control group; when the stimulation frequency increased continually,discharge frequencies of GPi / SNr neurons in both two groups decreased. When stimulus frequency was fixed,the excitatory response proportion of GPi / SNr in fatigue group was higher than that in control group（ P 〈0. 05）,the firing activity frequency of SNr neurons in fatigue group was significantly higher than that in control group（ P〈 0.05）,and the activity and inhibitory response frequency of SNr were higher than those of GPi. After microinjection with KA,the inhibitory response proportion of SNr unit in fatigue group was higher than that in control group,and also higher than that of GPi unit in fatigue group; after microinjection with SCH23390,the excitatory response proportion of SNr unit in fatigue group was higher than that in control group. Conclusions： As information output nerve nuclei,GPi / SNr plays different integration roles in direct and indirect pathways of signal processing. During exercise-induced fatigue condition,indirect pathway intervention increases high frequency stimulation threshold to induce GPi / SNr firing activity. Reducing GPi / SNr exciting input from STN may be a way to relieve exercise-induced fatigue.