中文摘要：

采用缩足反射观察椎管内注射感觉神经元特异性受体（SNSR）激动剂牛肾上腺髓质8-22肽（BAM8-22）对吗啡耐受的影响．结果表明．椎管内注射BAM8-22（O．1nm01）后，吗啡抗伤害作用的半数有效剂量由耐受时的80．99pg恢复至12．38pg（P〈0．001）；隔天混合给予BAM8－22后．能显著延缓吗啡耐受，第6天吗啡抗伤害作用的半数有效剂量为14．84μg，与吗啡耐受组相比有极显著差异（P〈0．001）；但这两组动物吗啡的抗伤害作用没有达到正常吗啡组水平（P〈0．05和P〈0．01）．连续椎管内单独注射BAM8－226d后，吗啡抗伤害作用的半数有效剂量为12．23μg，明显高于正常吗啡组（P〈0．01）．但又明显低于吗啡耐受组（P〈0．001）．而椎管内连续6d混合给予BAM8－22和吗啡，并不能阻止吗啡耐受．研究表明，椎管内注射BAM8－22能部分翻转或延缓吗啡的耐受，而BAM8－22的长期作用则会部分降低吗啡的抗伤害作用，提示感觉神经元特异性受体参与了阿片受体功能的调制。

英文摘要：

To investigate the effect of intrathecal （i. t.） administration of bovine adrenalmedulla 8-22 （BAM8-22）, an agonist of sensory neuron-specific receptors （SNSR）, on mor- phine tolerance by paw withdrawal test. It was found that i.t. administration of BAM8-22（0. 1 nmol） evidently reversed established morphine tolerance, half effective dose of mor- phine antinociception reduced from 80.99μg in morphine tolerance to 12.38μg Mter BAMS-22 reversed （P 〈 0. 001） . Co-administration of BAM8-22 （0. 1 nmol） every other day, but not daily, with morphine remarkably attenuated the development of morphine tolerance, halfeffective dose of morphine antinociception in sixth day was 14.84 μg （P〈0. 001） . But antinociception of these two groups didn＇t reach to morphine in naive group level （P〈0.05and P〈0. 01） . Half effective dose of morphine antinociception was 12.23 μg after i.t. ad- ministration of BAM8-22 （0. 1 nmol） daily for six days, it was significantly higher than mor-phine in naive group （P ,（0.05） , but evidently lower than morphine tolerant group （P〈0. 001） . Co-administration of BAMS-22 daily, with morphine didn＇t attenuate the develop-ment of morphine to lerance. The present study showed that BAM8-22 inhibited the develop-ment and expression of tolerance to morphine, while chronic BAM8-22 weakened antinoci- ceptive response of morphine. These results suggest that SNSR is involved in the modulation of opioid receptors function.