中文摘要：

通过结扎大鼠L5脊神经建立神经病理性痛模型.手术后第3日开始皮下注射酮色林（0.3 mg.kg-1）或其溶剂DMSO（体积分数为0.8%）,每d 1次,2周后灌流、取材,免疫组织化学实验显示神经结扎后L4背根神经节（DRG）和脊髓背角-层nNOS的表达均上调.然而皮下注射酮色林阻断5-HT2A受体,使L4 DRG和脊髓背角细胞中nNOS的表达大大减少.说明阻断外周5-HT2A受体后,能抑制神经病理性痛DRG和脊髓背角nNOS的表达上调.

英文摘要：

Neuropathic pain model was established by L5 spinal nerve ligation（SNL） in rats.Vehicle（0.8% DMSO） or ketanserin（0.3 mg·kg-1） was injected subcutaneously once per day starting on day 3 following the surgery for two weeks.Immunohistochemistry study revealed that the expression of neuronal nitric oxide synthase（nNOS） in L4 dorsal root ganglion（DRG） and NADPH-d-stained neurons in laminae Ⅰ-Ⅱ of the spinal cord dorsal horn were greatly increased following the surgery of SNL.However,injection of the 5-HT2A receptor antagonist ketanserin,but not vehicle,completely abolished the increase in nNOS or NADPH-d expression in the spinal cord and DRG.The results in present study suggest that blockade of 5-HT2A receptor in the periphery inhibited upregulation of nNOS at the spinal level.