中文摘要：

两性霉素B为多烯类抗真菌抗生素,可通过影响细胞膜通透性发挥抑制真菌生长的作用,被广泛用于治疗引起患者免疫缺陷的全身真菌感染性疾病。其药理作用对生物膜性质的影响一直是一个关注热点。作者利用Langmuir-Blodgett膜技术和原子力显微镜（atomic force microscope,AFM）分别研究了鞘磷脂/两性霉素B二元混合单层膜及鞘磷脂、胆固醇和两性霉素B三元混合单层膜体系的热力学特性和表面形貌。通过对二元混合系统和三元混合系统的表面压力-平均分子面积曲线的测量,定量分析了固定膜压下的平均分子面积、过量分子面积和过量吉布斯自由能等参量,并分别观测了二元混合单层膜和三元混合单层膜的形态。热力学分析结果表明,胆固醇的加入可以促进鞘磷脂/两性霉素分子之间的相互作用,同时,AFM观察结果表明,一定量的胆固醇可以提高混合单层膜的平整度和稳定性。

英文摘要：

Amphotericin B （AmB）, which is a polyene antibiotic, is widely used to treat systemic fungal infections immunodeficiency diseases by exerting effects on cellular permeability and inhibiting the fungal growth. It is still an important hot issue for studying the pharmacological function and effect on cell membranes. Thermodynamic properties and surface morphology were studied using Langmuir-Blodgett （LB） technique and atomic force microscopy （AFM） for sphingomyelin（SM）/AmB binary system and SM/AmB/ Cholesterol ternary mixed monolayers. By analyzing the surface pressure-mean molecular area isotherms of mixed monolayers, the authors obtained the mean molecular area, excess molecular area and excess Gibbs energy at a given surface pressure. Meanwhile, they observed the morphology of binary and ternary systems. The results showed that cholesterol could induce the interaction between SM and AmB stronger and make the monolayers more flat and stability.