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中文摘要：
采用超声和差速离心方法，研究了猪肝线粒体ATP合酶水溶性部分（F1-ATP合酶）的提取及检测方法．对初始提取液和纯化后的溶液分别进行酶比活力测量，并对常规聚丙烯酰胺电泳后的胶条进行胶内活性检测．结果表明，纯化后的F1-ATP合酶水解ATP比活力显著提高．
英文摘要：
The extraction and detection of F1-ATP synthase from pig-liver mitochondria are investegated by using ultrasonic and differential centrifugal methods. The specific activity of F1- ATP synthase in both primary and purified solutions are measured first, then the in-gel enzymatic anylysis are made after polyacrylamide gel electrophoresis. The results show that the specific activity of F1-ATP synthase in purified solution is increased compared with that of the primary solution.
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