为了研究不同强度的间歇性禁食对黄鳝生长、消化酶活性及血液生化指标的影响。在(25±1)℃水温条件下,以鲜活水蚯蚓为饵料设定5种投喂模式饲养黄鳝:第一种为连续投喂的对照组;第二种禁食1 d后再投喂1 d;第三种禁食2 d后再投喂2 d;第四种禁食4 d后再投喂4 d;第五种禁食8 d后再投喂8 d。5种方式分别以C、S1F1、S2F2、S4F4和S8F8表示,实验周期64 d。结果显示:(1)与对照组相比,遭受间歇性禁食的黄鳝的特定生长率(SGR)、相对增重率及终末体质量均显著性降低,但其实际摄食率(FR)却显著提升,且S1F1组的食物转换效率(FCE)显著高于其它各组。因而,不同强度的间歇性禁食均诱导出部分补偿生长效应,其中S1F1的部分补偿效应最强;(2)S1F1组胃组织的蛋白酶活性显著高于S2F2组,S1F1组和S2F2组胃组织的蛋白酶活性显著高于其它组;各实验组的胃、前肠、后肠和肝脏等组织的淀粉酶活性均与C组无显著差异;S1F1组肝脏组织和后肠组织的脂肪酶活性均显著高于C组、S4F4组和S8F8组;(3)各实验组血清的总蛋白含量和葡萄糖含量均无显著差异;C组与S1F1组之间的血清总胆固醇含量无显著差异,但却显著高于S2F2组、S4F4组和S8F8组;在S2F2组、S4F4组和S8F8组之间,其血清胆固醇含量随着间歇性禁食强度的增加而降低。实验结果表明,黄鳝在遭受间歇性禁食过程中,可通过相应消化酶活性的提升、食物利用率的改善以及消化生理机能的适应来获得生长补偿,且相对低强度的间歇性禁食(S1F1)可诱导出较强的补偿生长效应。
The experiment was carried out to investigate the effects of intermittent food-deprivation on growth,digestive enzyme activities and some serum biochemical indices in the rice-field eel, Monopterus albus. A 64-day growth trial with five feeding regimes was conducted using the live tubificid worms (Limnodrilus hoffmeisteri)as the food at(25 ± 1 )℃. Groups of fish were deprived of food for 1,2,4,8 day (s) and then followed by satiation re-feeding for the corresponding day (s), respectively (i. e. S1F1, S2F2, S4F4 and S8F8 groups). The fish fed to satiation daily throughout the experiment served as the control group (i. e. C ). The results showed that, deprived fish had significantly lower specific growth rates( SGR), relative weight gains and final body weights, but significantly higher actual feeding rates, than that of the control fish (P 〈 0.05 ). Moreover, the fish in S1F1 group had significantly higher feed conversion efficiency(FCE) than those of any other groups ( P 〈 0.05 ). It indicated that the partial growth compensation was elicited by various extents of intermittent food-deprivation, and that the fish of S1F1 group showed the best growth compensation. The protease activity of stomach in the S1F1 group was significantly higher than that in the S2F2 group ( P 〈 0.05 ), and the protease activities of stomach in both of the two groups were significantly higher than those in other groups ( P 〈 0.05 ). There was no difference in amylase activities of liver, stomach, foregut and hindgut among fish in all groups ( P 〉 0.05). In the liver and hindgut, the S1F1 group had significantly higher lipase activities than other groups of the control, the S4F4 or the S8F8 (P 〈 0.05). In all groups of the deprived fish, the serum total protein contents and glucose contents were slightly higher and lower than that in the control group, respectively, however, there was no significant difference among the five treatments( P 〉 0. 05 ). There was no s