为研究安徽长江水系黄鳝(Monopterus albus)的遗传多样性和群体遗传结构,测定了其6个地理群体(当涂、无为、繁昌、贵池、怀宁和望江)共178尾个体的线粒体DNA控制区部分序列。对其长度为556~558 bp的控制区同源序列进行分析,共检测到变异位点41个(变异率7.35%),单倍型56种。平均单倍型多样性和核苷酸多样性分别为0.694~0.954、0.00237~0.01382。群体分化指数(Fst)和基因流(Nm)分别为0.01974~0.87529、0.07124~24.82928,分子变异分析(AMOVA)中,群体间遗传变异占61.72%,表明黄鳝群体间具有明显的遗传分化。基于单倍型或群体间遗传距离的分子系统进化树均显示,6个地理群体分为两支:当涂与繁昌群体聚为一支,其余4群体聚为另一支。
To assess the genetic diversity and population structure of Monopterus albus, 178 individuals of 6populations(DT, WW, FC, GC, HN, WJ) from Anhui reaches in the Yangtze River were investigated based on 556-558 bp nucleotide sequences of mt DNA partial control region. The sequences analysis results showed that 41 variable sites(mutation rates of 7.35%), 56 haplotypes were detected. The haplotype diversity(Hd) and nucleotide diversity(Pi) were0.694-0.954 and 0.00237-0.01382, respectively. Analysis of molecular variance(AMOVA) revealed that genetic variation among the populations was 61.72%. These results indicated that there were significant genetic differentiations among populations. NJ-tree based on haplotypes or genetic distances among populations showed that DT and FC populations were clustered as one, and other 4 population was as another independent cluster.