中文摘要：

目的介绍一种在微动脉段标本上进行全细胞膜片钳记录的技术。方法在离体豚鼠耳蜗螺旋动脉（SMA）、小脑前下动脉（AICA）和肠系膜动脉（MA）分支微动脉（直径小于100μm）的原位平滑肌细胞上,应用全细胞膜片钳技术。结果微动脉段上平滑肌细胞静息膜电位平均值在-25～-37 mV之间,细胞膜电容（Cinput）平均值在70～250 pF之间,均高于消化分离的单个平滑肌细胞。应用缝隙连接阻断剂DPBA（100μmol/L）后,SMA、AICA和MA微动脉段上平滑肌细胞膜电阻（Rinput）分别为（4 937±741）MΩ（n=12）、（3 703±367）MΩ（n=8）和（3336±479）MΩ（n=12）,细胞Cinput分别为（4.5±0.2）pF（n=9）、（7.1±0.7）pF（n=5）和（9.6±0.9）pF（n=7）,与单个平滑肌细胞相似,表明微动脉细胞间存在着丰富的缝隙连接。结论微动脉段标本全细胞膜片钳记录技术适用于微动脉细胞间缝隙连接、神经递质和药物对微动脉作用机制的研究。

英文摘要：

Objective To introduce a method of whole-cell patch clamp recording from arteriolar segments.Methods Whole-cell patch clamp recordings were performed on smooth muscle cells（SMCs） in situ of isolated segments from guinea pig spiral modiolar arteries（SMA）,anterior inferior cerebellar artery（AICA） branches and mesenteric artery（MA） branches（100 μm in diameter）.Results The mean resting membrane potential of the three arterioles ranged from-25 mV to-37 mV,while the mean membrane capacitance（Cinput）of the three arterioles ranged from 70 pF to 250 pF,and both were much higher than those of the isolated single smooth muscle cell.After application of the gap junction blocker DPBA（100 μmol/L）,membrane resistance（Rinput） of cells in situ was（4 937±741） MΩ（n=12）,（3 703±367） MΩ（n=8） and（3 336±479） MΩ（n=12） in SMA,AICA and MA segments,respectively.The Cinput was（4.5±0.2） pF（n=9）,（7.1±0.7） pF（n=5） and（9.6±0.9） pF（n=7）,respectively.Both Rinput and Cinput were much closer to those of the isolated single smooth muscle cell,suggesting that gap junctions existed between cells of the arterioles.Conclusion Whole-cell patch clamp recording from cells in situ of arteriolar segments is potentially useful in the study of intercellular gap junctions and the mechanisms underlying the actions of neurotransmitters and drugs on arterioles.