中文摘要：

目的：建立新的土拨鼠α干扰素（IFN-α）基因分型方法，分析急性和慢性土拨鼠肝炎病毒（Woodchuck hepati-tis virus，WHV）感染的土拨鼠肝细胞中不同型别的IFN-α基因表达谱及其意义。方法：利用已知序列和基因型的土拨鼠IFN-α基因克隆质粒，建立基于土拨鼠IFN-α基因序列的限制性片段长度多态性的土拨鼠IFN-α基因分型方法。PolyIC体外刺激正常土拨鼠外周血单个核细胞（PBMC），调查正常土拨鼠PBMC受PolyIC刺激后IFN-α基因的表达谱。提取急性和慢性土拨鼠肝炎病毒感染的土拨鼠肝组织mRNA，RT-PCR扩增土拨鼠IFN-α基因，分子克隆后调查急性和慢性感染的土拨鼠肝细胞中IFN-α基因表达谱。结果：建立了土拨鼠IFN-α基因限制性片段长度多态性分型方法。急性和慢性感染的土拨鼠肝细胞中IFN-α基因的表达谱明显不同于正常动物的表达谱，假基因表达所占比例大。结论：新建立的土拨鼠IFN-α基因分型方法可用于分析土拨鼠不同组织中IFN-α的基因表达谱。

英文摘要：

Objective ：To establish a novel method of genotyping for woodchuck（ Marmota monax） interferon alpha（IFN-ot） to investigate the profdes of IFN-ot expressed in the liver of woodchuck with acute and chronic woodchuck hepatitis virus（WHV） infection. Methods： Analysing sequence data about different genotype woodchuck IFN-α and establishing a novel genotyping method for woodchuck IFN-α based on the restriction length polymorphism analysis. After drawing the total RNA of liver tissue with either acute or chronic WHV-infected, woodchuck IFN-α gene were amplified by RT-PCR and cloned. The profiles of woodchuck IFN-α gene expressed in the liver were analyzed. Naive woodchuck PBMCs were stimulated with high concentration PolyIC, and the expressing profiles of IFN--α were investigated. Results： A novel genotyping method for wIFN-α based on the restriction length polymorphism analysis was developed. Expression profiles of IFN-α in livers of woodchucks were changed after the animals were infected by WHV, and the pseudogenes made their majoricy. There was difference between expressing profiles of IFN-α in naive woodchuck liver and PBMC. Conclusion：The novel genotyping method for woodchuck IFN-α can be used to investigate the profiles of IFN-α expressed in different tissue of woodchuck infected by WHV. It will help for studying the action of different subtype IFN-α and the mechanism of antiviral of interferon.