中文摘要：

瞄准：为了调查 APOBEC3G 的效果，调停了对在房间文化和复制的肝炎 B (HBV ) 的抗病毒的活动能干的 HBV 基于向量的老鼠模型。方法：哺乳动物的肝细胞瘤房间 Huh7 和 HepG2 是有编码 APOBEC3G 和复制的驾驶 CMV 的表示向量的各种各样的数量的 cotransfected 能干 1.3 褶层在长度上 HBV。在 transfected 房间的媒介的 HBsAg 和 HBeAg 的层次被 ELISA 决定。在 transfected 房间的 HBcAg 的表示被西方的污点检测。从细胞内部的核心粒子的 HBV DNA 和 RNA 被北、南部的污点分析检验。估计 APOBEC3G 在活体内的活动，一个 HBV 基于向量的模型在哪个 APOBEC3G 和 HBV 向量经由大量的尾巴静脉注射被共同交付被使用。在重量的单位的 HBsAg 和 HBV DNA 的层次一象在老鼠的肝的 HBV 联系核心的 RNA 一样的 of 老鼠被 ELISA 和量的 PCR 分析分别地决定。结果：在细胞内部的联系核心的 HBV DNA 的层次和 HBsAg 和 HBeAg 的细胞外的生产有剂量依赖者减少。细胞内部的联系核心的病毒的 RNA 的层次也减少了，但是在 transfected 房间的 HBcAg 的表示没几乎显示出变化。与在试管内结果一致，在重量的单位的 HBsAg 的层次一 of 老鼠戏剧性地被减少。在浆液 HBV DNA 和肝 HBV RNA 的层次的超过 1.5 log10 减少与控制组相比在对待 APOBEC3G 的组被观察。结论：这些调查结果显示 APOBEC3G 能压制 HBV 复制和抗原表示在活体内和在试管内，在 HBV 感染的处理答应进展。

英文摘要：

AIM： To investigate the effect of APOBEC3G mediated antiviral activity against hepatitis B virus （HBV） in cell cultures and replication competent HBV vector-based mouse model. METHODS： The mammalian hepatoma cells Huh7 and HepG2 were cotransfected with various amounts of CMV-driven expression vector encoding APOBEC3G and replication competent 1.3 fold over-length HBV. Levels of HBsAg and HBeAg in the media of the transfected cells were determined by ELISA. The expression of HBcAg in transfected cells was detected by western blot. HBV DNA and RNA from intracellular core particles were examined by Northern and Southern blot analyses. To assess activity of the APOBEC3G in vivo, an HBV vector-based model was used in which APOBEC3G and the HBV vector were co-delivered via high-volume tail vein injection. Levels of HBsAg and HBV DNA in the sera of mice as well as HBV core-associated RNA in the liver of mice were determined by ELISA and quantitative PCR analysis respectively. RESULTS： There was a dose dependent decrease in the levels of intracellular core-associated HBV DNA and extracellular production of HBsAg and HBeAg. The levels of intracellular core-associated viral RNA also decreased, but the expression of HBcAg in transfected cells showed almost no change. Consistent with in vitro results, levels of HBsAg in the sera of mice were dramatically decreased. More than 1.5 log10 decrease in levels of serum HBV DNA and liver HBV RNA were observed in the APOBEC3G-treated groups compared with the control groups.CONCLUSION： These findings indicate that APOBEC3G could suppress HBV replication and antigen expression both in vivo and in vitro, promising an advance in treatment of HBV infection.