中文摘要：

目的：观察新的抗乙型肝炎病毒（HBV）核苷类似物——替比夫定体外对乙型肝炎病毒复制子的抑制作用。方法：将乙型肝炎病毒复制子pHBV1．3质粒转染Huh7细胞，在转染细胞培养液中加入不同浓度的替比夫定，然后在不同时间段观察替比夫定的抗病毒作用。ELISA检测培养上清中HBsAg、HBeAg的表达，荧光定量PCR（FQ-PCR）定量检测培养上清中HBVDNA拷贝数，Southern blot分析转染细胞中HBV复制中间体。结果：替比夫定减少了培养上清中HBSAg、HBeAg的表达及HBVDNA拷贝数，抑制了转染细胞中HBV复制中间体的形成。替比夫定对HBV复制子体外复制的抑制作用依赖于药物浓度及作用时间。结论：替比夫定在体外对HBV复制有较强的抑制作用。

英文摘要：

OBJECTIVE To observe antiviral effect of telbivudine, a novel antiviral nucleoside analogue, against HBV replicon in vitro. METHODS Hepatitis B virus replicon pHBV1.3 plasmid, a containing 1.3 copies of HBV genome was transfected into Huh7 cell line, and telbivudine were added to medium with different concentration post-transfection. The levels of HBsAg, HBeAg and HBV DNA in supernatant of Huh7 cells were measured by ELISA and FQ-PCR, respectively. Intracellular HBV replicative intermediates were analyzed by Southern blot. RESULTS Telbivudine inhibited HBV replication in Huh7 cells transfected with pHBV1.3. The levels of HBsAg, HBeAg and HBV DNA in supernatant of Huh7 cells treated with telbivudine were remarkably reduced, and intracellular HBV replicative intermediates were also decreased. The inhibition effect of telbivudine depended on the concentration drug and action time. CONCLUSION Telbivudine strongly inhibited HBV replication in huh7 cells transfected with pHBV1.3 plasmids, telbivudine can be further used in clinic for chronic hepatitis B therapy.