中文摘要：

对杂色鲍Haliotis diversicolor的离体血细胞进行不同浓度（10-3～10-9M）的重金属离子（Cd2＋、Hg2＋、Cu2＋和Zn2＋）胁迫,应用PI染色的流式细胞术测定血细胞活性的变化。结果显示胁迫6h后,10-3M和10-4MCd2＋,10-3M、10-4M和10-5MHg2＋,10-3MCu2＋,10-3M和10-4MZn2＋导致血细胞的死亡率显著上升（P〈0.05）;当浓度为10-3M时,Cd2＋、Hg2＋、Cu2＋和Zn2＋组的死亡率分别为41.76%、48.75%、23.28%和28.52%。四种重金属对杂色鲍血细胞的毒性依次为：Hg2＋〉Cd2＋〉Zn2＋〉Cu2＋。

英文摘要：

After in vitro exposure with 10-3～10-9 M heavy metals （Cd2＋, Hg2＋, Cu2＋ and Zn2＋）, cell viability of haemocytes from Haliotis diversicolor were determined using PI staining by flow cytometry. Results showed that after in vitro exposure for 6 h, 10-3M and 10-4M Cd2＋, 10-3M, 10-4M and 10-5M Hg2＋, 10-3M Cu2＋, 10-3M and 10-4M Zn2＋ significantly decreased the cell viability （P＆lt;0.05）. Mortality of H. diversicolor haemocytes were 41.76%, 48.75%, 23.28% and 28.52% after exposed to 10-3M Cd2＋, Hg2＋, Cu2＋ and Zn2＋ respectively. The toxicity of these four heavy metals on H. diversicolor haemocytes can be ranked in the following order： Hg2＋ ＆gt;Cd2＋＆gt; Zn2＋＆gt; Cu2＋.