中文摘要：

对凡纳滨对虾（Litopenaeusvannamei）血细胞进行离体镉离子（Cd^2＋）应激试验。cd^2＋浓度为10^3-10^-9M，在应激6h后取样，利用流式细胞术（FCM）检测血细胞的活性和活性氧（ROS）含量。结果显示，当cd^2＋浓度为10。M时，血细胞的ROS含量显著升高；当cd。’浓度为10。和10^-4M时，血细胞的死亡率和ROS含量均显著上升；表明Cd^2＋的细胞毒性作用与其浓度相关，cd^2＋浓度达到10^-5M时即可诱导血细胞ROS的产生，但当cd^2＋浓度达到10^-4M及以上时，被诱导产生的ROS对细胞造成氧化伤害，从而导致细胞死亡。

英文摘要：

Stress test of cadmium ion on haemocytes of Litopenaeus vannamei in vitro was conducted. Exposed to concentration of 10^-9 - 10^-3 M Cd^2＋ in 6 h in vitro, haemocyte viability and reactive oxygen species （ROS） of L. vannamei haemocyte samples were determined through flow cytometry （FCM）. The results showed that ROS con- tents in haemocytes surged significantly under the concentration of 10^-5 M cadmium; Under the concentration be- tween 10^-4 and 10^-3 M cadmium, mortality and ROS contents of haemocytes ascended emphatically respectively. These results showed the cytotoxicity of Cd^2＋ on L. vannamei was in relevance to the concentration of Cd^2＋. ROS of haemocytes would be induced with Cd^2＋ concentration reaching 10^-5 M, but the induced ROS would cause oxida- tive damage to haemocytes when Cd^2＋ concentration reaching 10^-4 M or above, thus contributing to haemocyte death.