中文摘要：

应用流式细胞术（FCM）对克氏原螯虾（Procambarusclarkii）血细胞的分类、活性和免疫功能进行了研究。结果显示：血细胞可分为透明细胞、小颗粒细胞和大颗粒细胞三个亚群，组成比例分别为（26．25±5．29）％、（51．44±7．02）％和（11．20±1．82）％；螯虾血细胞的平均总凋亡率约为3．12％；血细胞对荧光大肠杆菌的吞噬活力显著（P〈0．05）高于荧光微球，吞噬率分别为17．04％和14．57％；血细胞在自然生理状态下含有一定量的活性氧，其在两类颗粒细胞的含量显著（P〈0．05）高于透明细胞，在大颗粒细胞中最高。结果表明，FCM能较好地应用于虾类的血细胞分类和功能研究。

英文摘要：

Classification, viabihty and immune function of crayfish （Procambarus clarkii） haemocytes were. studied using flow cytometry. Results showed that haemocytes were divided into three subpopulations, including hyaline cells, semigran- ular cells and granular cells, with the proportions of （ 26. 25 ± 5. 29 ） % , （ 51.44 ± 7.02 ） % and （ 11.20 ± 1.82 ） %, re- spectively. The average apoptotic ratio was 3.12% in the total haemocytes. Phagocytic activity against fluorescent E. coli was significantly higher than that of fluorescent microsphere （ P 〈 0. 05 ）. The phagocytic ratios were 17.04 and 14.57%, re- spectively. Haemocytes also produced reactive oxygen species at the natural physiological state. The reactive oxygen produc- tion was significantly higher in the two granular cells than hyaline cells （ P 〈 0. 05 ）, and highest in granular cells. The present study showed the superiority of FCM on study of cellular classification and functions of shrimp haemocytes.