中文摘要：

以新鲜虾血细胞为阴性对照，α-羰基氰化氯苯腙处理的虾血细胞为阳性对照,建立以JC-1为荧光标记的测定虾类血细胞线粒体膜电位的流式细胞术方法。将凡纳滨对虾离体血细胞经不同浓度Cd^2＋（10-9～10-3mol／L）处理6h后，应用该方法测定血细胞线粒体膜电位的变化。结果显示10-4mol／L和10-3mol／LCd^2＋导致血细胞线粒体膜电位显著下降，表明该浓度的Cd^2＋会破坏虾血细胞线粒体的功能。本研究结果也显示，应用JC-1标记的流式细胞术测定方法适用于虾类血细胞线粒体膜电位的研究。

英文摘要：

A flow cytometric method for measurement of mitochondrial membrane electronic potential was established in haemocytes of Pacific white leg shrimp Litopenaeus vannamei using JC-1 staining. In the flow cytometric method, the fresh shrimp haemocytes were used for negative control, and the CCCP- treated haemocytes were tested for positive control. The mitochondrial membrane electronic potential were determined in the haemocytes of Pacific white leg shrimp exposed to different doses （10-9-10-3mol/L） of Cd2＋ for 6 h in vitro. Results showed that significant decrease in the mitochondrial membrane electronic potential was observed in the haemocytes exposed to 10-4mol/L and 10-3 mol/L Cd2＋ , indicating that these doses of Cd2＋ led to the disfunction of the haemocyte mitochondria. The present findings also demonstrated that the flow cytometric method with JC-1 staining was feasible for analysis of mitochondrial membrane electronic potential in shrimp haemocytes.