中文摘要：

目的了解不同培养基对福建金线莲种子萌发以及幼苗生长的影响。方法比较6种基本培养基对福建金线莲种子萌发的影响，观察并统计种子萌发率；利用正交设计研究萘乙酸（NAA）、6-苄基腺嘌呤（6-BA）对福建金线莲种子萌发形成的幼苗叶片数、茎节数、根数、根长度、株高、鲜重等的影响。结果不同基本培养基对福建金线莲种子萌发及原球茎分化效果不同，种子在MS培养基上萌发率最高，达60．38％，原球茎在1／2MS培养基上分化成幼苗效果最好；不同植物激素组合对福建金线莲幼苗生长影响无显著性差异，1／2MS＋NAA 1．0mg·L^-1＋6．BA2．0mg·L^-1效果较好。结论适宜的培养基能够促进福建金线莲种子的萌发与幼苗生长。

英文摘要：

OBJECTIVE To study the effect of different media on seed germination and young seedling propagation of Anoectochi- lus roxburghii. METHODS A. roxburghii seeds were cultured in 6 basal media for 12 weeks, and germination rates were calculated; orthogonal design was used to study the effects of NAA and 6-BA on the propagation of young seedlings of A. roxburghii. After 4 months, the leaf number, stem internode number, root number, the length of root, plant height, and fresh mass were measured respec- tively. RESULTS The effects of different media on seed germination and protocorm differentiation of A. roxburghii were different. Germination rate was the highest on MS medium, which was 60. 38%, and protocorm differentiation was optimal on half-strength MS medium. Different combinations of NAA and 6-BA didn＇ t show significant difference on the growth of young seedlings of A. roxburghii, and the appropriate medium was 1/2MS ＋ NAA 1.0 mg·L^-1 ＋ 6-BA 2. 0 mg· L^-1. CONCLUSION The optimization of culture medium can stimulate seed germination and young seedling propagation of A. roxburghii.