中文摘要：

目的将探索 paeoniflorin (PF ) 导致的推迟的 neuroprotection，在中国药规定的 Paeoniae 根值的主要部件，和它在受到脉管的痴呆(VD ) 的老鼠的内在的机制。VD 的一个老鼠模特儿被双边的普通颈动脉动脉吸藏(BCCAO ) 劝诱的方法。低剂量或高剂量的 PF (20 或 40 mg/kg 一次每天) 在 VD 以后被管理 28 天。老鼠的行为的分析走水路被测量莫利斯舞。地区性的服的血体积(rCBV ) ，地区性的服的血 flflow (rCBF ) 和吝啬的运输时间(MTT ) 被灌注加权的成像(PWI ) 在双边的马头鱼尾的怪兽测量。interleukin-1 (IL-1 ) 的层次， interleukin-6 (IL-6 ) ，和肿瘤坏死因素 alpha (TNF-) 被商业地可得到的连接酶的 immunosorbent 试金工具包测量。蛋白质层次被西方的污点分析评估。mRNA 层次被真实时间聚合酶链反应评估。西方的弄污被用来估计 p65 translocation。行为的分析显示出的结果那 PF 能减少逃跑潜伏时间(P < 0.05 ) ，并且增加原来的站台象限的住处时间并且到对面在在 VD 老鼠的水迷宫的站台频率(P < 0.05 ) 。同样， PF 显著地支持了 rCBV (P < 0.05 ) ， rCBF 并且每极小的 MTT 减少了(P < 0.05 ) 在 VD 老鼠的马头鱼尾的怪兽。而且， PF 减少了 IL-1， IL-6 和 TNF- 象一样的版本在 VD 老鼠的马头鱼尾的怪兽禁止了 IL-1， IL-6 和 TNF- 的 mRNA 表示(P < 0.05 或 P < 0.01 ) 。PF 能也减少在 VD 老鼠的马头鱼尾的怪兽的可诱导的氮的氧化物 synthase 和 cyclooxygenase-2 的蛋白质表情(P < 0.05 或 P < 0.01 ) 。另外， PF signifificantly 禁止了原子因素 B (NF-B ) 在 VD 老鼠的马头鱼尾的怪兽的小径。结论 PF signifificantly 稀释认知缺陷，改进马头鱼尾的怪兽灌注并且禁止在 VD 老鼠的 inflflammatory 反应。另外， PF 的 anti-inflflammatory 效果可能由于禁止 NF-B 小径。PF 可以是在改进 VD 的潜在的临床的应用。

英文摘要：

Objective： To explore the delayed neuroprotection induced by paeoniflorin（PF）, the principal component of Paeoniae radix prescribed in Chinese medicine, and its underlying mechanisms in rats subjected to vascular dementia（VD）. Methods： A rat model of VD was induced by bilateral common carotid arteries occlusion（BCCAO）. Low-dose or high-dose PF（20 or 40 mg/kg once per day） was administrated for 28 days after VD. The behavioral analysis of rat was measured by water morris. Regional cerebral blood volume（r CBV）, regional cerebral blood flow（r CBF） and mean transit time（MTT） were measured in the bilateral hippocampus by perfusion-weighted imaging（PWI）. The levels of interleukin-1β（IL-1β）, interleukin-6（IL-6）, and tumor necrosis factor alpha（TNF-α） were measured by commercially available enzyme-linked immunosorbent assay kits. Protein levels were evaluated by western blot analysis. m RNA levels were evaluated by real time-polymerase chain reaction. Western blotting was used to estimate p65 translocation. Results： The behavioral analysis showed that PF could decrease the escape latency time（P〈0.05）, and increase the residence time of the original platform quadrant and the across platform frequency in water maze in VD rats（P〈0.05）. Likewise, PF remarkably promoted the r CBV（P〈0.05）, r CBF and decreased per minute MTT（P〈0.05） in hippocampus of VD rats. Furthermore, PF decreased the release of IL-1β, IL-6 and TNF-α as well as inhibited the m RNA expression of IL-1β, IL-6 and TNF-α in the hippocampus of VD rats（P〈0.05 or P〈0.01）. PF also could decrease the protein expressions of inducible nitric oxide synthase and cyclooxygenase-2 in the hippocampus of VD rats（P〈0.05 or P〈0.01）. In addition, PF significantly inhibited the nuclear factor κB（NF-κB） pathway in the hippocampus of VD rats. Conclusions： PF significantly attenuates cognitive impairment, improves hippocampus perfusion and inhibits inflammatory response in VD ra