中文摘要：

建立了无胶筛分毛细管电泳-激光诱导荧光（Non-gel sieving capillary electrophoresis with laser inducedfluorescence,NGS-CE-LIF）检测脱氧核糖核酸（DNA）-蛋白质相互作用产物的方法。考察了筛分介质聚环氧乙烷（Poly（ethylene oxide）,PEO）浓度、分离温度、分离电压及缓冲液三羟甲基氨基甲烷硼酸（Tris-Borate-ED-TA,TBE）pH值等条件对pUC19 DNA/MspⅠ（HpaⅡ）DNA Marker和Protein Molecular Weight Marker（Broad）D532S相互作用产物的影响。结果表明,当PEO浓度为0.1%,TBE的pH为9.0,分离电压为15 kV,分离柱温15℃时,DNA-蛋白质相互作用产物得到有效分离。本方法应用于人胃癌组织p53基因扩增后的PCR产物和从相应组织中提取的蛋白质二者相互作用产物时,检测效率高,分离效果好。

英文摘要：

A method was established to separate DNA-protein interaction products by non-gel sieving capillary electrophoresis with laser induced fluorescence（NGS-CE-LIF）.A series of effects on CE were studied,namely sieving medium concentration,separation temperature,separation voltage,pH value of Tris-Borate-EDTA（TBE） buffer.The results indicated that pUC19 DNA/MspⅠ（HpaⅡ） DNA marker and protein molecular weight marker（Broad） D532S interaction products were effectively separated when sieving medium was 0.1%（w/V） poly（ethylene oxide）（300000）,electrophoresis buffer was 1×TBE（pH9.0）,separation voltage was 15 kV,and temperature was 15 ℃.The detection method of DNA-protein interaction products was used to analyze the interaction of the products of PCR of p53 gene and protein complex from gastric cancer tissue.The method has high detection rate and good separation efficiency.