中文摘要：

目的对广东省珠海市恙虫病东方体进行基因分型。方法予高热期恙虫病患者静脉采血后,立刻床旁小白鼠腹腔接种,待发病后解剖,取其腹壁刮液涂片、吉姆萨染色镜检;以巢式PCR进行恙虫病东方体DNA检测和序列分析,将结果进行同源性分析。结果床旁小白鼠腹腔接种,腹壁刮液涂片镜检可见紫红色圆形、椭圆形、短杆状恙虫病东方体菌体。10例恙虫病患者进行特异性的巢式PCR扩增,其中6例扩增出目的基因片段,阳性率为60%。将扩得珠海恙虫病东方体基因片段的DNA序列与基因库序列进行比对,发现其与韩国株Yonchon株同源性最高,大于95%,系统进化树显示其与Karp株在同一分支。结论珠海市为恙虫病的自然疫源地,该地区恙虫病东方体的基因型与Yonchon株最相近。

英文摘要：

The genotyping of disease caused Orientia tsutsugamushi in Zhuhai City,Guangdong Province,China was studied.We inoculated them to the abdominal cavity of laboratory rat immediately after collection of the venous blood of patients during febrile period.The autopsy was made after onset of tsutsugamushi disease followed by microscopic examining of the scraping smear of abdominal wall with Wright Giemsa staining.DNA detection and sequence analysis of Orientia tsutsugamushi were resorted to nested PCR.Results were administered by homology analysis.Mice inoculated intraperitoneally were found with purplish red circular,oval and short rod strain of Orientia tsutsugamushi in abdominal wall by scraping smear microscopic examination.Ten cases of tsutsugamushi disease patients were administered with specific nested PCR amplification,6out of which were amplified with target gene fragment with 60% of positive rate.By comparing the amplified DNA sequences with sequences of the gene pool,we found that its homology was the highest with the South Korean Yonchon strains for exceeding95%.The phylogenetic tree showed that it was in the same branch with the Karp strain.Zhuhai City is natural epidemic area of tsutsugamushi disease,and the genotype of Orientia tsutsugamushi in this area is the most similar with that of Yonchon strain.