中文摘要：

目的：建立常山胡柚3种主要黄酮类物质含量的测定方法,并比较常山胡柚中皮、肉、籽中该3种黄酮类物质含量的差异。方法：采用反相高效液相色谱法分离检测胡柚中柚皮苷、橙皮苷、新橙皮苷的含量;流动相为乙腈-水（23：77,V/V）,柱温25℃,流速1.0mL/min,检测波长283nm。结果：柚皮苷在3.22～1030μg/mL、橙皮苷在0.59～380μg/mL、新橙皮苷在0.077～990μg/mL范围内,黄酮类物质的质量浓度与峰面积呈良好的线性关系;柚皮苷、橙皮苷、新橙皮苷的平均回收率分别为98.43%（RSD=4.07%）、98.70%（RSD=4.30%）、99.90%（RSD=3.47%）;并以柚皮苷、橙皮苷和新橙皮苷为标准比较胡柚副产物皮、籽与胡柚肉含量的差异,初步得出皮〉肉〉籽。结论：该方法简便、准确、可靠、重复性好,可用于常山胡柚的质量控制。

英文摘要：

Objective： To establish a method for determining naringin,hesperidin,and neohesperindin in Citrus paradisi cv.Changshan Huyou fruit and compare their contents in different parts such as peel,pulp and seeds.Methods： The contents of naringin,hesperidin and neohesperindin were determined by a reversed-phase HPLC method.Methanol extracts of samples and the standard substances were separated on a YMC C18 column（4.6 mm × 250 mm,5μm） using a mobile phase made up of acetonitrile and water（23：77,V/V） at a flow rate of 1 mL/min and detected by a UV detector at a wavelength of 283 nm.The column temperature was set at 25 ℃.Results： The calibration curves were linear in the range of 3.22-1030μg/mL for naringin,0.59-380μg/mL for hesperidin,and 0.077-990μg/m for neohesperindin,respectively.The average recoveries of naringin,hesperidin and neohesperindin were 98.43%（RSD = 4.07%）,98.70%（RSD = 4.30%） and 99.90%（RSD = 3.47%）,respectively.The contents of naringin,hesperidin,neohesperindin were the highest in the peel and the lowest in the seeds of Citrus paradisi cv.Changshan Huyou fruit.Conclusions： This method is simple,accurate,reliable and reproducible and can therefore be used for the quality control of Citrus paradisi cv.Changshan Huyou fruit.