中文摘要：

目的：提取、分离、鉴定绞股蓝热处理产物中抑制肺癌A549细胞的活性成分。方法：绞股蓝叶在温度125℃、压力0.24MPa条件下,加热处理3h,用80%乙醇加热回流提取3h,利用活性跟踪方法,通过大孔树脂HP-20及反相柱等分离手段,分离抑制肺癌A549细胞的活性成分,并用核磁共振波普（1H-NMR、13C-NMR）、电喷雾质谱（ESI-MS）等数据鉴定有效成分。结果：绞股蓝热处理产物中分离得到2个达玛烷类皂苷,分别为绞股蓝皂苷Gypenoside L和Gypenoside LI,而且它们对肺癌A549细胞具有质量浓度依赖性的抑制活性（P〈0.05）,其IC50值分别为（48.43±1.33）μg/mL和（34.35±0.88）μg/mL。结论：热处理绞股蓝产物可提高对肺癌A549细胞的抑制作用,其中有效成分为达玛烷类皂苷Gypenoside L和Gypenoside LI。

英文摘要：

Objective： To extract,separate and identify anti-A549 cell constituents from thermally processed Gynostemma pentaphyllum leaves.Methods： Gynostemma pentaphyllum leaves were processed at 125 ℃ and 0.24 MPa for 3 h and subjected to heat reflux extraction with 80% ethanol for 3 h.Anti-A549 cell constituents in the extract were separated using HP-20 macroporous adsorption resin and a reversed-phase column by means of bioassay-guided separation and identified by1H-NMR,13C-NMR and ESI-MS.Results： Two dammarane-type saponins were obtained and identified as gypenoside L and gypenoside LI,respectively.Both gypenosides had a concentration-dependent inhibitory effect on A549 cells with IC50 values of（48.43 ± 1.33） μg/mL and（34.35 ± 0.88） μg/mL,respectively.Conclusion： Thermally processed Gynostemma pentaphyllum leaves have a notable inhibitory effect on A549 cells,which is associated with the presence of gypenoside L and gypenoside LI.