中文摘要：

建立应用nanoLC／MS／MS技术寻找羊水特异性表达蛋白质的蛋白质组学方法．羊膜穿刺获取两份正常妊娠晚期的羊水，通过Cibacron blue和Protein A吸附去除血清白蛋白和IgGs，样品经变性、还原和烷基化后酶解，nano LC／MS／MS分析，鉴定了62种蛋白质．通过与已有的羊水和血浆蛋白质表达数据库比对，从中新发现22种只在羊水中表达的蛋白质，其中4种已知是子宫或妊娠特异的．本研究建立了nano LC／MS／MS对羊水进行蛋白组学分析的方法并发现了一些可能的羊水特异表达蛋白．

英文摘要：

To establish proteome analysis methods for human amniotic fluid （AF） with nanoLC/MS/ MS for searching specific protein expressed in AF. Two specimens of normal AF were collected by transamniotic puncture, albumin and IgGs were depleted by Cibacron blue and protein A adsorption, then the protein samples were denatured, reduced, alkylated, digested and analyzed by nano LC/MS/MS. The proteins identified in the present study were compared with the proteins already identified in AF and human plasma. 62 proteins identified in the present study, among which 22 were the new found proteins exclusively presented in AF, 4 of them had already been described to be placenta or pregnancy specific, In the present study we establish proteome analysis methods for human amniotic fluid （AF） with nanoLC/ MS/MS and find several possible AF specific protein candidates.