中文摘要：

目的 探讨染料木黄酮（5，7，4’-三羟基异黄酮）对大鼠肋生长板软骨细胞（ratcosto-ehondral growth plate chondrocyte，RGC）胶原和Sox9表达的影响。方法 原代培养RGC，^3H-proline参人、RT-PCR和Westernblot分别检测染料木黄酮对第1代RGC胶原合成、col2al基因转录和Sox9表达的影响。结果 染料木黄酮抑制RGC的胶原合成（P〈0．05），col2al的转录和Sox9的表达，其抑制作用随着浓度的升高而增强。结论 染料木黄酮抑制培养RGC胶原合成的作用至少部分是通过下调Sox9的表达实现的。

英文摘要：

Aim To investigate effects of genistein （5, 7, 4＇-trihydroxyisoflavone） on rat costochondral growth plate chondrocyte （RGC） collagen and Sox9 expression. Methods Primary cultured RGC, effects of genistein on collagen synthesis, col2al mRNA transcription and Sox9 protein expression of 1st passage RGC were detected by isotope incorporation, RT-PCR and western blotting. Results Genistein inhibited collagen synthesis of 1st passage RGC （P 〈 0.05 ）,col2al mRNA transcription and Sox9 protein expression. The inhibitory effect of genistein enhanced with its increasing concentration. Conclusion The inhibitory effect of genistein on collagen synthesis of RGC is partially mediated by the down-regulation of Sox9 expression.